Main Title |
Screening Complex Hazardous Wastes for Mutagenic Activity Using a Modified Version of the TLC/Salmonella Assay. |
Author |
Houk, V. S. ;
Claxton, L. D. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;North Carolina Univ. at Chapel Hill. School of Public Health. |
Year Published |
1986 |
Report Number |
EPA/600/J-86/024; |
Stock Number |
PB86-187234 |
Additional Subjects |
Hazardous materials ;
Wastes ;
Bacteria ;
Toxicology ;
Bioassay ;
Reprints ;
Salmonella ;
Mutagenesis
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB86-187234 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
14p |
Abstract |
Ten complex hazardous wastes were tested for mutagenic activity using a modified version of the TLC/Ames assay developed by Bjorseth et al. (1982). This fractionation/bioassay scheme couples thin layer chromatography (TLC) with the Salmonella/mammalian-microsome (Ames) assay for the detection of mutagenic constituents in complex mixtures. Crude (unadulterated) hazardous wastes and selected hazardous waste extracts were fractionated on commercially available cellulose TLC plates. Mutagenicity testing was performed in situ by applying a single overlay of minimal growth agar, tester strain TA98 or TA100, and the optional metablic activation system directly onto the developed chromatogram. A mutagenic effect was indicated either by the appearance of localized clusters of revertant colonies or by an increase in total revertant growth vis-a-vis control plates. Seven of ten hazardous wastes (including tars, emulsions, sludges, and spent acids and caustics) demonstrated mutagenic activity when tested by this method. |
Supplementary Notes |
Pub. in Mutation Research 169, p81-92 1986. Prepared in cooperation with North Carolina Univ. at Chapel Hill. School of Public Health. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Screening Complex Hazardous Wastes for Mutagenic Activity Using a Modified Version of the TLC/Salmonella Assay. |
PUB Date Free Form |
c1986 |
Category Codes |
6T; 57Y |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
617120159 |
Origin |
NTIS |
Type |
CAT |