Main Title |
R-Plasmid Transfer to and from 'Escherichia coli' Strains Isolated from Human Fecal Samples. |
Author |
Corliss, T. L. ;
Cohen, P. S. ;
Cabelli, V. J. ;
|
CORP Author |
Health Effects Research Lab., Cincinnati, OH. |
Year Published |
1981 |
Report Number |
EPA-600/J-81-226; |
Stock Number |
PB81-212151 |
Additional Subjects |
Escherichia coli ;
Bacteria ;
Transferring ;
Humans ;
Feces ;
Reprints ;
Plasmids ;
NTISEPAORD
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB81-212151 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
10p |
Abstract |
Strains of Escherichia coli recently isolated from human feces were examined for the frequency with which they accept and R factor (Ri) from a derepressed fi+ strain of E. coli K-12 and transfer it to fecal and laboratory strains. Colicins produced by some of the isolates rapidly killed the other half of the mating pair; therefore, conjugation was conducted by a membrane filtration procedure whereby this effect was minimized. The majority of fecal E. coli isolates accepted the R factor at lower frequencies than K-12 F-, varying from 10-2 per donor cell to undetectable levels. The frequencies with which certain fecal recipients received the R-plasmid were increased when its R+ transconjugant was either cured of the R1 plasmid and remated with the fi+ strain or backcrossed into the parental strain. The former suggests the loss of an incompatibility plasmid, and the latter suggests the modification of the R1-plasmid deoxyribonucleic acid (DNA). In general, the fecal R+ E. coli transconjugants were less effective donors for K-12 F- and heterologous fecal strains than was the fi+ K-12 strain, whereas the single strain of Citrobacter freundii examined was generally more competent. Passage of the R1-plasmid to strains of salmonellae reached mating frequencies of 10-1 per donor cell when the recipient was a Salmonella typhi previously cured of its resident R-plasmid. However, two recently isolated strains of Salmonella accepted the R1-plasmid from E. coli K-12 R+ or the R+ E. coli transconjugants at frequencies of 5x10-7 or less. |
Supplementary Notes |
Pub. in Applied and Environmental Microbiology, v41 n4 p959-966 Apr 81. Prepared in cooperation with Rhode Island Univ., Kinston. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: R-Plasmid Transfer to and from 'Escherichia coli' Strains Isolated from Human Fecal Samples. |
PUB Date Free Form |
29 Jan 81 |
Category Codes |
6M; 57K |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
323799514 |
Origin |
NTIS |
Type |
CAT |