Main Title |
Transfection with Baculovirus DNA. |
Author |
Burand, John P. ;
Summers, Max D. ;
Smith, Gale E. ;
|
CORP Author |
Texas A and M Univ., College Station. Dept. of Entomology.;Health Effects Research Lab., Research Triangle Park, NC. |
Year Published |
1980 |
Report Number |
EPA-R-805232-01; EPA-600/J-80-257; |
Stock Number |
PB81-191959 |
Additional Subjects |
Viruses ;
Deoxyribonucleic acid ;
Molecular biology ;
Microorganisms ;
Cells(Biology) ;
Reprints ;
Autographa californica ;
Rachiplusia ou ;
Transfection
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB81-191959 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
8p |
Abstract |
Purified DNA from the nuclear polyhedrosis viruses of Autographa californica (AcM NPV) and Rachiplusia ou (RoM NPV) were found to be infectious in TN-368 cells employing the calcium phosphate precipitation technique (F.L. Graham and A.J. van der Eb, Virology, 52, 456-467, 1973). Transfection with AcMNPV produced 3600 PFU/microgram DNA compared to 2900 PFU/microgram DNA with RoMNPV. Of eight baculovirus DNAs tested, only AcMNPV DNA and RoMNPV DNA could transfect TN-368 cells. The in vitro host range of AcMNPV DNA was determined to be the same as AcMNPV extracellular virus. AcMNPV Form 1 DNA was fourfold more infectious in TN-368 cells than Form II DNA. |
Supplementary Notes |
Pub. in Jnl. of Virology 101, p286-290 1980. Errata sheet inserted. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Transfection with Baculovirus DNA. |
PUB Date Free Form |
c1980 |
Category Codes |
6M; 6C; 57K; 57F |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
323797138 |
Origin |
NTIS |
Type |
CAT |