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OLS Field Name OLS Field Data
Main Title Changes in Accessibility of DNA to Various Fluorochromes during Spermatogenesis.
Author Evenson, D. ; Darzynkiewicz, Z. ; Jost, L. ; Janca, F. ; Ballachey, B. ;
CORP Author South Dakota State Univ., Brookings. Dept. of Chemistry. ;Sloan-Kettering Inst. for Cancer Research, New York. Lab. of Investigative Cytology.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1986
Report Number EPA/600/J-86/163;
Stock Number PB87-114609
Additional Subjects Deoxyribonucleic acid ; Flow cytometry ; Reprints ; Fluorochromes ; Spermatogenesis
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NTIS  PB87-114609 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 11p
Abstract Accessibility of mouse testicular and vas deferens (vas) sperm cell DNA to acridine orange, propidium iodide, ellipticine, Hoechst 33342, mithramycin, chromomycin A3, 4'6-diamidino-2-phenylindole (DAPI), and 7-amino-actinomycin D (7-amino-AMD) was determined by flow cytometry. Permealized cells were either stained directly or after pretreatment with 0.06 N HCI. For histone-containing tetraploid, diploid, and round spermatid cells, HCI extraction of nuclear proteins caused an approximately sixfold increase of 7-amino-AMD stainability but had no significant effect on DAPI stainability. For these same cell types, the stainability with other intercalating (acridine orange, propidium iodide, ellipticine) and externally binding (Hoechst 33342, mithramycin, chromomycin A3) dyes was increased by 1.6- to 4.0-fold after HCI treatment. In sharp contrast, HCI treatment of vas sperm did not increase the staining level of 7-amino-AMD, DAPI, or propidium iodide but did increase the staining level for the other intercalating dyes (1.3- to 1.5-fold) and external dyes (1.3- to 1.9-fold).
Supplementary Notes Pub. in Cytometry 7, p45-53 Jan 86. Prepared in cooperation with Sloan-Kettering Inst. for Cancer Research, New York. Lab. of Investigative Cytology. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
NTIS Title Notes Journal article.
Title Annotations Reprint: Changes in Accessibility of DNA to Various Fluorochromes during Spermatogenesis.
PUB Date Free Form c1986
Category Codes 57F
NTIS Prices PC A02/MF A01
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 703715103
Origin NTIS
Type CAT