Main Title |
Methodology for Assessing Respiration and Cellular Incorporation of Radiolabeled Substrates by Soil Microbial Communities (Journal Version). |
Author |
Dobbins, D. C. ;
Pfaender, F. K. ;
|
CORP Author |
North Carolina Univ., Chapel Hill. Dept. of Environmental Sciences and Engineering.;Robert S. Kerr Environmental Research Lab., Ada, OK. |
Publisher |
c1988 |
Year Published |
1988 |
Report Number |
EPA/600/J-88/065; |
Stock Number |
PB89-103188 |
Additional Subjects |
Soil microbiology ;
Soil analysis ;
Kinetics ;
Biodeterioration ;
Metabolism ;
Respiration ;
Radioactivity ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB89-103188 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
19p |
Abstract |
A method is described for determining biodegradation kinetics of both naturally occurring and xenobiotic compounds in surface and subsurface soil samples. The method measures both respiration and uptake into cellular biomass of 14C-labeled substrates. After separation of the cells and the soil particles by centrifugation, the cells were trapped on membrane filters for liquid scintillation counting. Mass balances were easily obtained. The technique was used to measure metabolic activity in soil profiles, including unsaturated and saturated zones. First order rate constants were determined for amino acid metabolism and for m-cresol metabolism. Saturation kinetics were observed for amino acids and m-cresol. m-Cresol values for uptake often exceeded those for respiration by greater than a factor of ten. Saturation was not observed in many horizons. Frequently, respiration obeyed saturation kinetics, whereas uptake was first order. It is concluded that measuring only kinetics of respiration may lead to severe underestimations of biodegradation rates. (Copyright (c) Springer-Verlag New York Inc., 1988.) |
Supplementary Notes |
Pub. in Microbial Ecology, v15 n3 p257-273 May 88. Sponsored by Robert S. Kerr Environmental Research Lab., Ada, OK. |
NTIS Title Notes |
Journal article. |
Category Codes |
68E; 48E; 98A |
NTIS Prices |
PC A03/MF A01 |
Primary Description |
600/15 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
834421049 |
Origin |
NTIS |
Type |
CAT |