| Main Title |
Complex Frameshift Mutations Mediated by Plasmid pKM101: Mutational Mechanisms Deduced from 4-Aminobiphenyl-Induced Mutation Spectra in Salmonella. |
| Author |
Levine, J. G. ;
Schaaper, R. M. ;
DeMarini., D. M. ;
|
| CORP Author |
Environmental Protection Agency, Research Triangle Park, NC. Genetics Toxicology Div. ;North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering. ;National Inst. of Environmental Health Sciences, Research Triangle Park, NC. Lab. of Molecular Genetics. |
| Publisher |
c1994 |
| Year Published |
1994 |
| Report Number |
EPA/600/J-94/407; |
| Stock Number |
PB95-125399 |
| Additional Subjects |
Frameshift mutation ;
Plasmids ;
Salmonella ;
Mutagens ;
Alleles ;
Base sequence ;
DNA repair ;
SOS response(Genetics) ;
Polymerase chain reaction ;
Dose-response relationships ;
Reprints ;
4-aminobiphenyl
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB95-125399 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
20p |
| Abstract |
The authors used colony probe hybridization and polymerase chain reaction/DNA sequence analysis to determine the mutations in approximately 2,400 4-aminobiphenyl (4-AB) + S9-induced revertants of the -1 frameshift allele hisD3052 and of the base-substitution allele hisG46 of Salmonella typhimurium. Most of the mutations occurred at sites containing guanine, which is the primary base at which 4-AB forms DNA adducts. A hotspot mutation involving the deletion of a CG or GC within the sequence CGCGCGCG accounted for 100 and 99.9%, respectively, of the reversion events at the hisD3052 allele in the pKM101 plasmid-minus strains TA1978 (uvr(+)) and TA1538 (Delta uvrB). In strain TA98 (DeltauvrB, pKM101), which contained the SOS DNA repair system provided by the pKM101 plasmid, approximately 85% of the revertants also contained the hotspot deletion; the remaining approximately 15% contained one of two types of mutations: (1) complex frameshifts that can be described as a -2 or +1 frameshift and an associated based substitution and (2) deletions of the CC or GG sequences that flank the hotspot site (CCGCGCGCGG). The authors propose a misincorporation/slippage model to account for these mutations in which (1) pKM101-mediated misincorporation and translesion synthesis occurs across a 4-AB-adducted guanine; (2) the instability of such a mispairing and/or the presence of the adduct leads to strand slippage in a run of repeated bases adjacent to the adducted guanine; and (3) continued DNA synthesis from the slipped intermediate produces a frameshift associated with a base substitution. (Copyright (c) 1994 by the Genetics Society of America.) |
| Supplementary Notes |
Pub. in Genetics 136, p731-746 Mar 94. Prepared in cooperation with North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering. and National Inst. of Environmental Health Sciences, Research Triangle Park, NC. Lab. of Molecular Genetics. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Complex Frameshift Mutations Mediated by Plasmid pKM101: Mutational Mechanisms Deduced from 4-Aminobiphenyl-Induced Mutation Spectra in Salmonella. |
| Category Codes |
57F; 57K; 57Y |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
200/04 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
435317890 |
| Origin |
NTIS |
| Type |
CAT |
