| Main Title |
Simplified Radioimmunoassay for Detection of Human Rotavirus in Stools. |
| Author |
Cukor, George ;
Berry, Mary Kay ;
Blacklow, Neil R. ;
|
| CORP Author |
Massachusetts Univ. Medical School, Worcester.;Health Effects Research Lab., Cincinnati, OH. |
| Year Published |
1981 |
| Report Number |
EPA-R-805169 ;EPA-R-806591-01-0; EPA/600/J-78/184; |
| Stock Number |
PB81-231110 |
| Additional Subjects |
Diagnosis ;
Humans ;
Infants ;
Electron microscopy ;
Reprints ;
Radioimmunoassay ;
Rotaviruses
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB81-231110 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
7p |
| Abstract |
A simplified radioimmunoassay (RIA) technique was developed to facilitate the diagnosis of human rotavirus in stools of infants with diarrhea. This microtiter solid-phase RIA utilizes as a critical reagent hyperimmune serum against a tissue culture-grown simian rotavirus that is immunologically closely related to the human agent. One hundred thirteen pediatric stool samples were tested. Thirty-five specimens known to be positive for rotavirus by electron microscopy were also positive by RIA. All RIA-positive reactions (36) were shown to be specific for rotavirus by means of an antibody-blocking test. Nonspecific RIA-positive reactions were not encountered. This accurate, sensitive RIA technique is practical for use in diagnostic laboratories because critical serum reagents are prepared against culture-grown virus and not against rotavirus derived from carefully selected stools of humans or experimentally infected gnotobiotic animals. |
| Supplementary Notes |
Pub. in The Journal of Infectious Diseases, v138 n6 p906-910 Dec 78. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Simplified Radioimmunoassay for Detection of Human Rotavirus in Stools. |
| PUB Date Free Form |
c1981 |
| Category Codes |
6E; 57E |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
323801702 |
| Origin |
NTIS |
| Type |
CAT |
