Main Title |
Differential Cytotoxic Sensitivity in Mouse and Human Cell Lines Exposed to Organophosphate Insecticides. |
Author |
Veronesi, B. ;
Ehrich, M. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div. ;Virginia-Maryland Regional Coll. of Veterinary Medicine, Blacksburg, VA. |
Publisher |
1993 |
Year Published |
1993 |
Report Number |
EPA/600/J-94/039; |
Stock Number |
PB94-137155 |
Additional Subjects |
Organophosphate insecticides ;
Toxicity ;
Cell line ;
Species diversity ;
Cell survival ;
Mice ;
Humans ;
Esterases ;
Cytochrome P-450 ;
Dose-response relationships ;
Reprints ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB94-137155 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
7p |
Abstract |
Neuroblastoma cell lines were used to examine the differential interspecies response (i.e., species selectivity) to organophosphates (OPs). Baseline activities of the major target esterases, i.e., cholinesterase, carboxylesterase, and neurotoxic esterase, were assayed in mouse and several human neural candidate cell lines. These activities were found to be variable within individual cell lines and among the various tested cell lines. Cytotoxicity data using the neutral red fluorometric assay were collected on both human (SH-SY5Y) and mouse (NB41A3) neuroblastoma clones exposed to a variety of OP insecticides. IC50 data indicated that the tested mouse cell line was consistently more sensitive than the human cell line to equimolar doses of various OP compounds (e.g., mipafox, parathion, paraoxon, DFP, leptophos oxon, fenthion, and fenitrothion). These data suggest that interspecies-selectivity in response to OP-related cytotoxicity is influenced by intercellular differences in metabolism and baseline esterase activities. |
Supplementary Notes |
Pub. in Toxicology and Applied Pharmacology, v120 n2 p240-246 Jun 93. Prepared in cooperation with Virginia-Maryland Regional Coll. of Veterinary Medicine, Blacksburg, VA. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Differential Cytotoxic Sensitivity in Mouse and Human Cell Lines Exposed to Organophosphate Insecticides. |
Category Codes |
57Y; 57B; 68E |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
407719982 |
Origin |
NTIS |
Type |
CAT |