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Main Title Fiber Optic Fluoroimmunosensor for the Detection of Polychlorinated Biphenyls.
Author Rogers, K. R. ; Van Emon, J. M. ; Kline, R. ; Wright, J. ; Zhao, Q. ;
CORP Author Maryland Univ. at Baltimore.;Environmental Monitoring Systems Lab., Las Vegas, NV.
Publisher Feb 95
Year Published 1995
Report Number EPA-CR-820460; EPA/600/A-95/058;
Stock Number PB95-218855
Additional Subjects Fluorometers ; Chlorinated organic compounds ; DNA adducts ; Reprints ; Fiber optics ; Technology innovation ; Analytical chemistry ; Field tests ; Optical measuring instruments ; Fluorescence spectroscopy ; Immunoassay ; Pollution monitoring ; Hazardous materials ; Toxic substances ; Fluoroimmunosensor ; PCBs(Polychlorinated biphenyls) ; Anti-PCB serum ; Evanescent wave fluorometer
Library Call Number Additional Info Location Last
NTIS  PB95-218855 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
Abstract There currently exists a critical and growing need for cost-effective, real-time, and in situ methods for detection of environmental pollutants such as polychlorinated biphenyls (PCBs). Herein reported is a evanescent wave Fluoroimmununosensor for measurement of PCBS. 2,4-5-Trichlorophenoxybutyric acid (TCPB) was cross linked to fluorescein ethyleneamine (TCPB-FL) and used as a optical signal generator. Protein A purified IgG, obtained from anti-PCB serum, was immobilized on quartz fibers and used as the biological recognition element in an evanescent wave fluorimeter. The optical signal was generated by the binding of the fluorescent probe TCPB-FL to the antibody (Ab)-coated fiber. Fluorescent probe concentrations in the nM range resulted in reversible binding. Several Aroclors displaced the fluorescent probe (TCPB-FL) in a concentration-dependent manner. Arochlor 1248 was detected from the ppb to ppm range. Arochlor 1242 was also measured in methanol extracts from standard reference material soils.
Supplementary Notes Presented at the International Symposium on Field Screening Methods for Hazardous Wastes and Toxic Chemicals (4th), Las Vegas, NV., February 22-25, 1995. Sponsored by Environmental Monitoring Systems Lab., Las Vegas, NV.
Highlights Notes %AUT:M. E. /Eldefrawi
Title Annotations Reprint: Fiber Optic Fluoroimmunosensor for the Detection of Polychlorinated Biphenyls.
Category Codes 68G; 68; 99A; 57E; 57B; 57U
NTIS Prices PC A02/MF A01
Primary Description 600/07
Document Type NT
Cataloging Source NTIS/MT
Control Number 521404130
Origin NTIS
Type CAT