| Main Title |
Modified Agar Medium for Detecting Environmental Salmonellae by the Most-Probable-Number Method. |
| Author |
Hussong, D. ;
Enkiri, N. K. ;
Burge, W. D. ;
|
| CORP Author |
Agricultural Research Service, Beltsville, MD. Biological Waste Management and Organic Resources Lab. ;Maryland Conservation Service, Beltsville.;Environmental Protection Agency, Cincinnati, OH. Water Engineering Research Lab. |
| Year Published |
1984 |
| Report Number |
EPA/600/J-84/313; |
| Stock Number |
PB85-225308 |
| Additional Subjects |
Culture media ;
Salmonella ;
Bacteria ;
Microorganisms ;
Detection ;
Cultures(Biology) ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB85-225308 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
7p |
| Abstract |
Salmonellae in the environment remain a potential source of disease. Low numbers of salmonellae have been detected and enumerated from environmental samples by most probable number methods that require careful colony selection from plated agar medium. A modified xylose lysine brilliant green medium was prepared to control the loss of selectivity caused by heating the brilliant green component. Added agar reduced colony spreading. The medium contained 47 g of xylose lysine agar base per liter; the agar content was adjusted to 2%, autoclaved, cooled to 50 degrees C, and then amended just before pouring to include H2S indicator and 7 ppm (7 ml of 1:1,000 brilliant green per liter) of unheated brilliant green dye. H2S-positive salmonellae were easily detected from sewage sludge compost to the exclusion of most other gram-negative bacteria. |
| Supplementary Notes |
Prepared in cooperation with Maryland Conservation Service, Beltsville. Errata sheet inserted. |
| Availability Notes |
Pub. in Applied and Environmental Microbiology 48, n5 p1026-1030 Nov 84. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Modified Agar Medium for Detecting Environmental Salmonellae by the Most-Probable-Number Method. |
| PUB Date Free Form |
c1984 |
| Category Codes |
6M; 6F; 57K; 68D |
| NTIS Prices |
Not available NTIS |
| Primary Description |
600/14 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
526922553 |
| Origin |
NTIS |
| Type |
CAT |
