Main Title |
Immunochemical protocols / |
Other Authors |
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Publisher |
Humana Press, |
Year Published |
1992 |
OCLC Number |
24870840 |
ISBN |
0896032043; 9780896032040; 0896032701; 9780896032705 |
Subjects |
Immunochemistry--Methodology ;
Antibodies--isolation & purification--https://idnlmnihgov/mesh/D000906Q000302 ;
Immunochemistry--methods--https://idnlmnihgov/mesh/D007120Q000379 ;
Immunochimie ;
Techniques immunologiques ;
Immunochemie ;
Wetenschappelijke technieken ;
Immunchemie--(DE-588)4161366-1 ;
Methode--(DE-588)4038971-6 ;
Immunochimie--Technique ;
Aufsatzsammlung
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Internet Access |
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Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
EKCM |
QR183.6.I43 1992 |
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CEMM/GEMMD Library/Gulf Breeze,FL |
02/18/1994 |
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Collation |
xiii, 480 pages : illustrations ; 24 cm. |
Notes |
Includes bibliographical references and index. |
Contents Notes |
Production of polyclonal antisera -- Efficient elution of purified proteins from polyvinylidene difluoride membranes (immobilon) after transfer from SDS-PAGE and their use as immunogens -- Raising polyclonal antibodies using nitrocellulose-bound antigen -- Synthesis of peptides for use as immunogens -- Production and characterization of antibodies against synthetic peptides -- Preparation and testing of monoclonal antibodies to recombinant proteins -- Screening of monoclonal antibodies using antigens labeled with acetylcholinesterase -- Purification of immunoglobulin G (IgG) -- Epitope mapping -- Enzyme-antienzyme method for immunohistochemistry -- Double label immunohistochemistry on tissue sections using alkaline phosphatase and peroxidase conjugates -- Immunohistochemical detection of bromodeoxyuriding-labeled nuclei for in vivo cell kinetic studies -- Avidin-biotin technology: preparation of biotinylated probes -- Avidin-biotin technology: preparation of avidin conjugates -- Immunochemical applications of avidin-biotin technology -- Preparation of gold probes -- Immunogold probes for light microscopy -- Immunogold probes in electron microscopy -- Electron microscopic silver enhancement for double labeling with antibodies raised in the same species -- Quantitative and qualitative immunoelectrophoresis -- Rocket immunoelectrophoresis techniques or electroimmunodiffusion -- Crossed immunoelectrophoresis -- Crossed immunoaffinoelectrophoresis -- Immunodetection of proteins by western blotting -- Erasable western blots -- Colloidal gold staining and immunoprobing on the same western blot -- Colloidal gold staining and immunodetection in 2-D protein mapping -- Fluorescent protein staining on nitrocellulose with subsequent immunodetection of antigen -- Competitive ELISA -- Twin-site ELISAs for fos and myc oncoproteins using the AMPAK system -- Preparation of cytotoxic antibody-toxin conjugates -- Immunoaffinity purification and quantification of antibody-toxin conjugates -- An immuno-slot-blot assay for detection and detection and quantitation of alkyldeoxyguanosines in DNA -- Production of monoclonal antibodies for the detection of chemically modified DNA -- Sensitive competitive enzyme-linked immunoassay for quantitation of modified bases in DNA -- An immunochemical assay for detecting transition of B-DNA to Z-DNA -- Cell sorting using immunomagnetic beads -- Cell preparation for flow cytometry -- Preparation of rat lung cells for flow cytometry -- The isolation of rat hepatocytes for flow cytometry -- Flow cytometric analysis of cells using an antibody to a surface antigen -- Multiple immunofluorescence analysis of cells using flow cytometry -- Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine -- Production and use on nonradioactive hybridization probes -- Cellular human and viral DNA detection by nonisotopic in situ hybridization -- Chromosomal mapping of genes by nonisotopic in situ hybridization -- Nonisotopic in situ hybridization -- Biotinylated probes in colony hybridization -- Screening of agt11 cDNA libraries using monoclonal antibodies -- Expression of foreign genes in mammalian cells using an antibody fusion system. |
Place Published |
Totowa, N.J. : |
Title Ser Add Ent |
Methods in molecular biology (Clifton, N.J.) ; |
PUB Date Free Form |
c1992. |
Series Title Untraced |
Methods in molecular biology ; |
BIB Level |
m |
Cataloging Source |
OCLC/T |
LCCN |
91041416 //r92 |
Merged OCLC records |
29319524 |
OCLC Time Stamp |
19940217154639 |
Language |
ENG |
Origin |
OCLC |
Type |
CAT |
OCLC Rec Leader |
04341cam 2200325 a 45020 |