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Main Title Interlaboratory Validation Study Results for 'Cryptosporidium' Precision and Recovery for U.S. EPA Method 1622.
CORP Author Environmental Protection Agency, Washington, DC. Office of Water.
Publisher Apr 2001
Year Published 2001
Report Number EPA/821/R-01/027;
Stock Number PB2002-108711
Additional Subjects Cryptosporidium ; Filtration ; Microorganisms ; Design ; Objectives ; Laboratory tests ; Implementation ; Data reporting ; Data analysis ; Quality control ; Pathogens ; Parasitic diseases ; Drinking water ; Water analysis ; Laboratories ; EPA Method 1622 ; Interlaboratory validation
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NTIS  PB2002-108711 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 40p
Abstract This report presents the results of the U.S. Environmental Protection Agency's (EPA's) interlaboratory validation study (the 'Study') of Method 1622: Cryptosporidium by Filtration/IMS/FA (the 'Method'). The purpose of this Study was to determine the precision and recovery for Cryptosporidium in reagent water and raw surface matrices in multiple laboratories using the Method. One referee laboratory and 12 participating laboratories were involved in the validation Study. The Study was conducted in August 1998. The referee laboratory used a flow cytometer to sort a known number of unstained Cryptosproidium into spiking suspension containers. Six single-blind spiking suspensions and one double-blind reagent water blank were distributed to each participating laboratory. Each spiking suspension contained approximately 99 oocysts. Each laboratory analyzed four spiked reagent water samples, one reagent water blank, two spiked raw surface water samples, and one unspiked raw surface water sample according to the December 1997 version of the Method (EPA-821-R-97-023), as amended by technical clarifications. These clarifications have been incorporated in the final version of the Method.
Supplementary Notes See also PB2002-108709.
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Category Codes 68D; 57K; 99A
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Cataloging Source NTIS/MT
Control Number 029500740
Origin NTIS
Type CAT