| Main Title |
DNA Isolation from Small Tissue Samples Using Salt and Spermine. |
| Author |
Ross, J. A. ;
Nelson, G. B. ;
Holden, K. L. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. |
| Publisher |
c1991 |
| Year Published |
1991 |
| Report Number |
EPA/600/J-93/115; |
| Stock Number |
PB93-181048 |
| Additional Subjects |
Deoxyribonucleic acids ;
Spermine ;
Tissues(Biology) ;
Purification ;
Lymphocytes ;
Phenols ;
Chloroform ;
Solvents ;
Precipitin tests ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB93-181048 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
4p |
| Abstract |
Common DNA isolation methods rely upon protein denaturation by organic solvents such as phenol and chloroform. These solvents pose some risk to the user and require special disposal procedures. The authors have previously reported a method for isolating DNA from peripheral blood lymphocytes by precipitation with spermine. This procedure was less effective with other tissues, especially lung, and the spermine precipitates often took several days to dissolve in buffer. The authors have modified their procedure to include a pre-extraction of proteins with concentrated NaCl, essentially as described by Miller et al. This current, improved procedure provides good recovery of purified DNA even with small samples (< 200 mg) and a variety of tissue and cell types. |
| Supplementary Notes |
Pub. in Nucleic Acids Research Jnl., v19 n21 p6053-6054 Nov 91. Prepared in cooperation with Environmental Health Research and Testing, Inc., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: DNA Isolation from Small Tissue Samples Using Salt and Spermine. |
| Category Codes |
57F; 57B |
| NTIS Prices |
PC A01/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
316134214 |
| Origin |
NTIS |
| Type |
CAT |
