Main Title |
Determination of Absorbed Dose of Ozone (O3) in Animals and Humans Using Stable Isotope (Oxygen-18) Tracing. |
Author |
Hatch, G. E. ;
Aissa, M. ;
|
CORP Author |
Northrop Services, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. |
Year Published |
1987 |
Report Number |
EPA-68-02-4032; EPA/600/D-87/139; |
Stock Number |
PB87-191706 |
Additional Subjects |
Ozone ;
Absorption ;
Isotopic labeling ;
Dosimeters ;
Laboratory animals ;
Toxicology ;
Respiration ;
Mathematical models ;
Air pollution ;
Photochemical oxidants ;
Health risk
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB87-191706 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
26p |
Abstract |
A method for the determination of absorbed dose of ozone (03) in animals and humans using oxygen-18 (18)O as a physiological tracer is presented. The experimental aspects of the method are based on the instantaneous pyrolysis of tissue samples and subsequent conversion of the sample oxygen to carbon monoxide then to carbon dioxide whose isotopic composition is determined by isotope-ratio mass spectrometry. A mathematical procedure is then used to correct the isotopic data for interferences from the blank and memory effects and from the iodine pentoxide oxidation of CO to CO2. Laboratory animals were exposed to (18)O3 (1 ppm, 1 hr) then tissues were dried and processed for (18)O measurement. Enrichments in (18)O over natural abundance (18)O was observed in lung homogenates, nasal cavities, trachea, and pulmonary lavage fluids but not in blood of mice, rats, and rabbits. Thus, the (18)O tracing method appears to be sensitive enough to detect the reaction products of (18)O in animals exposed to near environmental concentrations of this gas. |
Supplementary Notes |
Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
PUB Date Free Form |
May 87 |
Category Codes |
68A; 68G; 57Y |
NTIS Prices |
PC A03/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
721923167 |
Origin |
NTIS |
Type |
CAT |