| Main Title |
On the Role of Metallothionein in Cadmium Absorption by Rat Jejunum In situ. |
| Author |
Kello, Dinko ;
Sugawara, Naoki ;
Voner, Cathleen ;
Foulkes, Ernest C. ;
|
| CORP Author |
Cincinnati Univ., OH. Coll. of Medicine.;Health Effects Research Lab., Cincinnati, OH. |
| Year Published |
1979 |
| Report Number |
EPA-600/J-79-143; |
| Stock Number |
PB82-127887 |
| Additional Subjects |
Cadmium ;
Absorption ;
Metals ;
Males ;
Rats ;
Laboratory animals ;
Exposure ;
Ingestion(Biology) ;
Jejunum ;
Digestive system ;
Reprints ;
Metallothionein ;
Drinking water
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB82-127887 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
12p |
| Abstract |
The role of metallothionein (MT) in the mechanism of cadmium absorption from the jejunum was studied in 7-9-week-old-male rats exposed to 50 ppm of cadmium in drinking water for 9 days. Exposed animals contained an average of 144 micrograms MT/g of mucosal tissue, compared to 40 micrograms in control animals. During jejunal perfusion in situ with 4 MM glucose-saline containing 10-20 nM CdCl2 the increased MT content of mucosa exerted no effect either on cadmium absorption from the lumen (step I), or on its further transport into the body (step II). Immediately after perfusion, essentially all cadmium removed from the lumen was fully recovered in the intestinal mucosa. About 50% of the mucosal cadmium was found in the sediment after homogenization and centrifugation; a large portion of this cadmium in the mucosal cytosol was not restricted to low molecular weight protein, although cadmium binding capacity in the MT fraction of controls as well as of exposed animals greatly exceeded actual binding of newly absorbed cadmium. Our results offer no support for the view that MT in the jejunal mucosa serves as determinant of cadmium absorption. |
| Supplementary Notes |
Pub. in Toxicology 14, p199-208 1979. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: On the Role of Metallothionein in Cadmium Absorption by Rat Jejunum In situ. |
| PUB Date Free Form |
7 Nov 79 |
| Category Codes |
6P; 57S |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
323807886 |
| Origin |
NTIS |
| Type |
CAT |
