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Main Title 'Giardia' Detection in Water Systems.
Author Sauch, J. F. ;
CORP Author Health Effects Research Lab., Cincinnati, OH.
Year Published 1986
Report Number EPA/600/D-86/234;
Stock Number PB87-104493
Additional Subjects Giardia ; Water supply ; Gastro intestinal diseases ; Detection ; Immune serums ; Cysts ; Identifying ; Biological stains ; Fluorescence ; Cell morphology ; Chlorination ; Tests ; Pennsylvania ; Massachusetts ;
Library Call Number Additional Info Location Last
NTIS  PB87-104493 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
Abstract A method has been developed combining immunofluorescence and phase-contrast to microscopically locate and identify Giardia cysts among particulates filtered from water samples. Selective staining of cysts with anti-Giardia antiserum and a fluorochrom enhances the contrast between cysts and other sample constituents and allows quick location of cysts by their green fluorecence. Their identity can then be confirmed with phase-contrast observation of size, shape, and internal morphology. The method was successfully tested during recent outbreaks in Pennsylvania and Massachusetts. However, when chlorinated water samples were tested, confirmation of presumptive Giardia cysts was difficult or impossible.
PUB Date Free Form Oct 86
Category Codes 57K; 57U; 48B
NTIS Prices PC A02/MF A01
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 635314807
Origin NTIS
Type CAT