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Main Title Immunoassay for p-Nitrophenol in Urine (Chapter XX).
Author Rogers, K. R. ; Van Emon, J. M. ;
CORP Author Environmental Monitoring Systems Lab., Las Vegas, NV.
Publisher 1993
Year Published 1993
Report Number EPA/600/A-93/074;
Stock Number PB93-175552
Additional Subjects Nitrophenols ; Urinalysis ; Immunoassay ; Enzyme-linked immunosorbent assay ; Occupational exposure ; Organophosphate insecticides ; Biological markers ; Fluorescence spectometry ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB93-175552 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
Abstract Urinary excretion of nitrophenol metabolites is an important index of human exposure to organophosphate pesticides. In particular, p-nitrophenol, a major urinary metabolite of parathion, can be used as a biomarker of human exposure. Immunoassay methods have been recently described for detection of p-nitrophenol. In the present paper, the effects of a urine matrix on the detection of p-nitrophenol are reported using both fluorescence and absorbance-based ELISAs. A competition format using the enzyme-amplified catalysis of 4-nitrophenyl phosphate or 4-methylumbelliferyl phosphate was used to report the analyte concentration. The presence of urine during the antibody-analyte interaction inhibited product formation in the final step of the assay and shifted the inhibition curves to the right, increasing the apparent I50 values for p-nitrophenol. The various urine samples collected from volunteers, not occupationally exposed to parathion, varied in their ability to inhibit color information and increase I50 values.
Title Annotations Reprint: Immunoassay for p-Nitrophenol in Urine (Chapter XX).
Category Codes 57D; 57Y; 57U; 68E
NTIS Prices PC A03/MF A01
Primary Description 600/07
Document Type NT
Cataloging Source NTIS/MT
Control Number 316130673
Origin NTIS
Type CAT