| Main Title |
Probabilistic View of the Transformation of Cultured C3H/10T1/2 Mouse Embryo Fibroblasts by 3-Methylcholanthrene (Cell Transformation/Chemical Carcinogenisis). |
| Author |
Fernandez, Alberto ;
Mondal, Sukdeb ;
Heidelberger, Charles ;
|
| CORP Author |
University of Southern California, Los Angeles.;Health Effects Research Lab., Cincinnati, OH. |
| Year Published |
1981 |
| Report Number |
EPA-R-805208; EPA/600/J-80-309; |
| Stock Number |
PB81-231094 |
| Additional Subjects |
Methylcholanthrene ;
Toxicity ;
Carcinogens ;
Mice ;
Embryos ;
Probability theory ;
Transformations ;
Reprints ;
Fibroblasts ;
Carcinogenesis
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB81-231094 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
8p |
| Abstract |
When C3H/10T1/2 cells are treated with a given concentration of a chemical carcinogen, the transformation frequency can vary over 4 orders of magnitude, depending primarily upon the number of cells plated. To explain this phenomenon, the authors have developed a probabilistic theory of the formation of transformed foci in this system. The authors define P1 as the probability that a cell will be activated by carcinogen treatment, P2 as the probability per cell generation that an activated cell will be transformed, and P3 as the probability per cell generation that an activated cell will be deactivated. The equation we have derived: log (F/N) = log (2p1p2(1-p3)/2(1-p3)-1) + n log(1-p3) describes focus formation; F is mean number of foci per dish after carcinogen treatment, N is number of cells in a dish at confluence, and n is number of cell generations to confluence. This equation has been verified experimentally; p3=0.24 and p1p2=3.8X10 to the minus 6 power at a single concentration of 3-methylcholanthrene. This relationship explains previously inexplicable effects of cell density on transformation frequency. |
| Supplementary Notes |
Pub. in Proceedings National Academy of Science, v77 n12 p7272-7276 Dec 80. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Probabilistic View of the Transformation of Cultured C3H/10T1/2 Mouse Embryo Fibroblasts by 3-Methylcholanthrene (Cell Transformation/Chemical Carcinogenisis). |
| PUB Date Free Form |
Jul 81 |
| Category Codes |
6T; 57Y |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
323801686 |
| Origin |
NTIS |
| Type |
CAT |
