Main Title |
Cytochemical Methods for Assessing 'Giardia' Cyst Viability. |
Author |
Lindmark, D. G. ;
|
CORP Author |
Cleveland State Univ., OH. Dept. of Biology.;Health Effects Research Lab., Research Triangle Park, NC. |
Year Published |
1987 |
Report Number |
EPA-R-811949; EPA/600/1-87/011; |
Stock Number |
PB88-124748 |
Additional Subjects |
Giardiasis ;
Parasitic diseases ;
Gastrointestinal diseases ;
Epidemiology ;
Toxicology ;
Air pollution ;
Water pollution ;
Hazardous wastes ;
Cytochemistry ;
Toxic substances ;
Environmental monitoring
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB88-124748 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
69p |
Abstract |
Giardia lamblia is the most common human intestinal protozoan parasite reported in the United States and England. The objective of the study was to develop a practical, reliable, rapid, microscopically-read method, comparable to excystation for determining the viability of Giardia cysts. At first an attempt was made to use methods dependent on cyst metabolism such as the activity of pyruvate: ferredoxin oxidoreductase (PFOR) and cell respiration. Though they gave promising results with trophozoites, they proved unreliable with cysts. Hence, used was a fluorescent dye exclusion technique previously proved to be successful for measuring viability in mammalian cells. |
Supplementary Notes |
Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
NTIS Title Notes |
Rept. for 23 Sep 84-22 Dec 86. |
PUB Date Free Form |
Oct 87 |
Category Codes |
57U; 57E; 68A; 68D |
NTIS Prices |
PC A04/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
802919722 |
Origin |
NTIS |
Type |
CAT |