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OLS Field Name OLS Field Data
Main Title Sequential Inoculation as an Adjunct in Enteric Virus Plaque Enumeration.
Author Benton, W. H. ; Hurst., C. J. ;
CORP Author Health Effects Research Lab., Cincinnati, OH. Toxicology and Microbiology Div.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/230;
Stock Number PB91-117085
Additional Subjects Inoculation ; Sewage ; Reprints ; Plaque assay ; Enteric viruses ; Cell line ; Idoxuridine ; Echoviruses ; Poliovirus
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB91-117085 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 03/04/1991
Collation 7p
Abstract The potential utility of sequentially inoculating a virus sample onto two different cultures of similar vs dissimilar cell lines was evaluated in conjunction with IDU (5-iodo-2'-deoxyuridine) treatment of the cells as a potential adjunct in viral plaque formation assays. This evaluation was done using laboratory grown human echovirus 7, human enterovirus 69 and human poliovirus 1, plus an environmental concentrate derived from sewage that contained indigenous untyped enteroviruses. The cell lines employed were BGM, RD, L-132 and HEL-299. Sequential inoculation generally yielded higher viral assay titers when compared with the more traditional method of simply introducing viral inoculum onto a culture of the first (initial) cell line and then completing the assay without removing that inoculum. When a permissive cell line (BGM or RD) was used for both the initial and final cultures in a sequential inoculation technique, the total plaque count titer from both the initial plus final cultures represented an average 35% improvement over the traditional method.
Supplementary Notes Pub. in Water Research, v24 n7 p905-909 Jul 90.
NTIS Title Notes Journal article.
Title Annotations Reprint: Sequential Inoculation as an Adjunct in Enteric Virus Plaque Enumeration.
Category Codes 57K
NTIS Prices PC A02/MF A01
Primary Description 600/10
Document Type NT
Cataloging Source NTIS/MT
Control Number 101123892
Origin NTIS
Type CAT