Main Title |
UAF Radiorespirometric Protocol for Assessing Hydrocarbon Mineralization Potential in Environmental Samples. |
Author |
Brown, E. J. ;
Resnick, S. M. ;
Rebstock, C. ;
Luong, H. V. ;
Lindstrom, J. ;
|
CORP Author |
Environmental Research Lab., Gulf Breeze, FL. ;Alaska Univ., Fairbanks. Inst. of Northern Engineering. ;Technical Resources, Inc., Gulf Breeze, FL. |
Publisher |
c1992 |
Year Published |
1992 |
Report Number |
EPA/600/J-92/389; |
Stock Number |
PB93-121218 |
Additional Subjects |
Land pollution control ;
Oil pollution ;
Water pollution control ;
Biodeterioration ;
Remedial action ;
Hydrocarbons ;
Oil spills ;
Crude oil ;
Microorganisms ;
Sediments ;
Mineralization ;
Beaches ;
Biomass ;
Nitrogen ;
Phosphorus ;
Carbon dioxide ;
Isotopic labeling ;
Performance evaluation ;
Reprints ;
Exxon Valdez ;
Radiorespirometry ;
Cleanup operations
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB93-121218 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
9p |
Abstract |
Following the EXXON Valdez oil spill, a radiorespirometric protocol was developed at the University of Alaska Fairbanks (UAF) to assess the potential for microorganisms in coastal waters and sediments to degrade hydrocarbons. The use of bioremediation to assist in oil spill cleanup operations required microbial bioassays to establish that addition of nitrogen and phosphorus would enhance biodegradation. A technique assessing 1-14C-n-hexadecane mineralization in seawater or nutrient rich sediment suspensions was used for both of these measurements. Hydrocarbon-degradation potentials were determined by measuring mineralization associated with sediment microorganisms in sediment suspended in sterilized seawater and/or marine Bushnell-Haas broth. Production of 14CO2 and CO2 was easily detectable during the first 48 hours with added hexadecane levels ranging from 10 to 500 mg/l of suspension and dependent on the biomass of hydrocarbon degraders, the hydrocarbon-oxidation potential of the biomass and nutrient availability. In addition to assessment of the hydrocarbon-degrading potential of environmental samples, the radiorespirometric procedure, and concomitant measurement of microbial biomass, has utility as an indicator of hydrocarbon contamination of soils, aqueous sediments and water, and can also be used to evaluate the effectiveness of bioremediation treatments. (Copyright (c) 1991 Kluwer Academic Publishers.) |
Supplementary Notes |
Pub. in Biodegradation, v2 p121-127 1991. Prepared in cooperation with Alaska Univ., Fairbanks. Inst. of Northern Engineering, and Technical Resources, Inc., Gulf Breeze, FL. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: UAF Radiorespirometric Protocol for Assessing Hydrocarbon Mineralization Potential in Environmental Samples. |
Category Codes |
68D; 68; 57K; 97R; 97K |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/04 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
300826319 |
Origin |
NTIS |
Type |
CAT |