| Main Title |
Quantification in Soil of 'Bacillus thuringiensis' var. 'kurstaki' delta-Endotoxin from Transgenic Plants. |
| Author |
Palm, C. J. ;
Donegan, K. ;
Harris, D. ;
Seidler, R. J. ;
|
| CORP Author |
Corvallis Environmental Research Lab., OR. ;ManTech Environmental Technology, Inc., Corvallis, OR. |
| Publisher |
c1994 |
| Year Published |
1994 |
| Report Number |
EPA/600/J-94/344; |
| Stock Number |
PB94-210515 |
| Additional Subjects |
Transgenic plants ;
Genetic engineering ;
Soil ;
Toxins ;
Biotechnology ;
Pest control ;
Plant genetics ;
Proteins ;
Plant ecology ;
Cotton plants ;
Biological insect control ;
Assaying ;
Reprints ;
Bacillus thuringiensis kurstaki delta-endotoxin ;
Btk toxin ;
Bacillus thuringiensis toxin ;
Bt toxin
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB94-210515 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
10p |
| Abstract |
Transgenic plants that produce pesticidal proteins have the potential to release these products into the environment when the plants are incorporated into soil. This could result in novel exposure of soil organisms to these pesticidal proteins. There is a lack of knowledge about the fate and persistence of transgenic pesticidal products in the soil. A model system of transgenic cotton, which produces Bacillus thuringiensis kurstaki (delta)-endotoxin (Bt toxin), was used to address this issue. Methods were developed to quantify Btk toxin in soil and soil/plant litter by extraction of the Btk toxin with an aqueous buffer and quantification by ELISA. The highest recovery of Btk toxin from soil was obtained with a high salt, high pH buffer. The method was shown to be useful in tracking over time the persistence of both purified and transgenic Btk toxin in laboratory experiments. |
| Supplementary Notes |
Pub. in Molecular Ecology 3, p145-151 1994. Prepared in cooperation with ManTech Environmental Technology, Inc., Corvallis, OR. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Quantification in Soil of 'Bacillus thuringiensis' var. 'kurstaki' delta-Endotoxin from Transgenic Plants. |
| Category Codes |
98D; 57P; 57F; 57K; 68 |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/02 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
428724570 |
| Origin |
NTIS |
| Type |
CAT |
