| Main Title |
Evaluation of Techniques for Mutagenicity Screening of Petroleum Samples. |
| CORP Author |
American Petroleum Inst., Washington, DC.;Environmental Protection Agency, Washington, DC. Office of Toxic Substances. |
| Publisher |
16 Aug 1982 |
| Year Published |
1982 |
| Report Number |
FYI-AX-0982-0130-SU; |
| Stock Number |
OTS-0000130-1 |
| Additional Subjects |
Mutagenicity ;
Screening ;
Petroleum ;
Detection ;
Solubilization ;
Sonication ;
Assays ;
Toxicity ;
Mutagenicity screening ;
Technique evaluation ;
Petroleum samples ;
Ames assay
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
OTS-0000130-1 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
873p |
| Abstract |
Simple modification of the Ames assay were evaluated for detecting mutagenicity of petroleum samples which are incompatible with the aqueous medium due to insolubility. Five petroleum sample were assayed using the standard plate incorporation Ames assay protocol. Results of these assays were invalid due to insolubility or separation of sample in the top agar layer. Solubilization of tar samples in DMSO was achieved by sonication and emulsions of liquid samples were prepared by vortexing. Such solubilized/emulsified samples also separated from the top agar overlay in a standard plate incorporation assay. Preincubation of sample and target organism in the presence of sonication (tar samples) or end-over-end rotation (liquid samples) in solutions/emulsion, permitted detection of mutagenicity of two samples and enhanced toxicity of a third. A sixth sample, tested as a gas in glass chambers, was found to be toxic but not mutagenic at the doses tested. |
| Supplementary Notes |
The quality of the documents listed in the Office of Toxic Substances database may not meet usual NTIS standards but are included to further the opportunity for the scientific and technical community to locate materials which may not be available elsewhere. The content is the responsibility of OTS. Prepared in cooperation with American Petroleum Inst., Washington, DC. |
| Availability Notes |
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| Category Codes |
99 |
| NTIS Prices |
PC A99/MF$25.00 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
010001750 |
| Origin |
NTIS |
| Type |
CAT |
