| Main Title |
Detection of Rotavirus with a New Polyclonal Antibody Enzyme Immunoassay (Rotazyme 2) and a Commercial Latex Agglutination Text (Rotalex): Comparison with a Monoclonal Antibody Enzyme Immunoassay. |
| Author |
Doern, G. V. ;
Herrmann, J. E. ;
Henderson, P. ;
Stobbs-Walro, D. ;
Perron, D. M. ;
|
| CORP Author |
Massachusetts Univ. Medical Center, Worcester.;Health Effects Research Lab., Research Triangle Park, NC. |
| Year Published |
1986 |
| Report Number |
EPA-R-812550; EPA/600/J-86/016; |
| Stock Number |
PB86-177813 |
| Additional Subjects |
Viruses ;
Detection ;
Humans ;
Feces ;
Bioassay ;
Reprints ;
Rotaviruses ;
Immunoassay ;
Agglutination test
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB86-177813 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
6p |
| Abstract |
A total of 176 human fecal specimens were examined for the presence of rotavirus using four different assays: a monoclonal antibody enzyme immunoassay; the original polyclonal antibody enzyme immunoassay marketed by Abbott Laboratories, Chicago, IL (Rotazyme I); a modification of this assay which is now commercially available (Rotazyme II); and a latex agglutination test (Rotalex) recently introduced by Medical Technology Corporation, Somerset, NJ. In addition, selected specimens were examined for the presence of rotavirus by electron microscopy, immune electron microscopy and by RNA gel electrophoresis. Forty specimens were positive using the monoclonal antibody enzyme immunoassay; 136 were negative. Using the results obtained with the procedure as the reference standard, the sensitivities of the Rotazyme I, Rotazyme II and Rotalex tests were 97.4, 100, and 81.6% respectively. The specificities of these three procedures were 88.8, 83.9, and 100%, respectively. |
| Supplementary Notes |
Pub. in Jnl. of Clinical Microbiology, v23 n2 p226-229 Feb 86. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Detection of Rotavirus with a New Polyclonal Antibody Enzyme Immunoassay (Rotazyme 2) and a Commercial Latex Agglutination Text (Rotalex): Comparison with a Monoclonal Antibody Enzyme Immunoassay. |
| PUB Date Free Form |
c1986 |
| Category Codes |
6E; 6M; 57E; 57K |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
614223848 |
| Origin |
NTIS |
| Type |
CAT |
