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RECORD NUMBER: 225 OF 298

Main Title Reoviruses in water pollution testing : final report /
Author Spendlove, Rex S. ; Barnett, B. B. ; George, D. B. ; Adams, D. J. ; Ridinger, D. N.
CORP Author Utah State Univ., Logan.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher U.S. Environmental Protection Agency,
Year Published 1984
Report Number EPA/600/1-84/022; EPA-R-806527
Stock Number PB85-125847
Subjects Reoviruses ; Sewage
Additional Subjects Reoviruses ; Water pollution ; Tests ; Sewage ; Enzymes ; Recovery ; Bioassay ; Tolerances(Physiology) ; Protamines ; Precipitation(Chemistry) ; Evaluation ; Abundance ; Safety ; Rotoviruses
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB85-125847 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 59 pages ; 28 cm
Abstract Twelve cell lines were tested to determine their sensitivity to reoviruses of three serotypes that had been isolated from sewage. Madin-Darby bovine kidney (MDBK) cells were the most susceptible. Sewage-isolated, protamine-precipitated reoviruses were also used in conjunction with MDBK cells in a comparative evaluation of immunofluorescent cell count (ICC) and plaque assay procedures. Immunofluorescence assay is more sensitive and more rapid than plaque assay procedure for quantifying reoviruses recovered from sewage. A procedure for protamine sulfate precipitation of reoviruses and rotaviruses was developed. Some reoviruses recovered from sewage were inactivated by enzyme treatment. This report describes a study to evaluate reoviruses for their potential usefulness in water quality testing.
Notes Caption title. "Final Report." "Nov. 1984." "EPA-600/1-84-022." Microfiche.
Place Published Washington, D.C. :
Corporate Au Added Ent Health Effects Research Laboratory (Research Triangle Park, N.C.) ; Utah State University.
PUB Date Free Form 1984.
NTIS Prices PC A04/MF A01
BIB Level m
Medium microform
Cataloging Source OCLC/T
OCLC Time Stamp 20011107164922
Language eng
Origin NTIS
Type MERGE
OCLC Rec Leader 01027cam 2200301Ka 45170