Main Title |
Distributional Kinetics of Ni2(+) in the Rat Lung. |
Author |
Williams, S. J. ;
Holden, K. M. ;
Sabransky, M. ;
Menzel, D. B. ;
|
CORP Author |
Duke Univ. Medical Center, Durham, NC.;Health Effects Research Lab., Research Triangle Park, NC. |
Year Published |
1980 |
Report Number |
EPA-R-806337; EPA-600/J-80-073; |
Stock Number |
PB81-184772 |
Additional Subjects |
Nickel ;
Kinetics ;
Lung ;
Rats ;
Air routes ;
Concentration(Composition) ;
In vivo analysis ;
Reprints ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB81-184772 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
10p |
Abstract |
The kinetics of removal of Ni2(+) from the airways of the rat lung was studied in vivo and ex vivo. The uptake of Ni2(+) from the vasculature was investigated ex vivo. For ex vivo studies, the isolated, ventilated, and perfused lung (IVPL) was prepared using negative pressure ventilation and was perfused with Krebs-Henseleit buffer solution at 37C. Rates of removal of Ni2(+) from the airways were determined following intratracheal instillation of 1, 10, or 127 nmol 63NiCl2 in 100 microliters of isotonic sucrose. The Ni2(+) remaining in the lung was calculated from the 63Ni2(+) activity which appeared in the perfusate. Ex vivo studies revealed a concentration dependent alteration in lung permeability which was not observed in vivo. Also the rate of removal of Ni2(+) from the airways was inhibited by the presence of Ca2(+) and Mg2(+) in the perfusate. Uptake of Ni2(+) from the vasculature was studied in the IVPL supplied with NiCl2 at 0.01, 0.1, 1.0, and 10 micromole/min. The net rate of Ni2(+) varied with the supply rate and did not appear to be saturable. A two-compartment model described the efflux of Ni2(+) from the vasculature. These studies suggest that the airways of the mammalian lung are largely impermeable to inhaled Ni2(+) in the presence of physiologic concentrations of divalent cations, while uptake from the vasculature is relatively rapid. |
Supplementary Notes |
Pub. in Toxicology and Applied Pharmacology 55, p85-93 1980. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: The Distributional Kinetics of Ni2(+) in the Rat Lung. |
PUB Date Free Form |
7 Apr 80 |
Category Codes |
6T; 6E; 57Y; 57O |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
323796086 |
Origin |
NTIS |
Type |
CAT |