| Main Title |
Hydroxyl Free Radical Mediated Formation of 8-Hydroxyguanine in Isolated DNA (Journal Version). |
| Author |
Floyd, R. A. ;
West, M. S. ;
Eneff, K. L. ;
Hogsett, W. E. ;
Tingey, D. T. ;
|
| CORP Author |
Oklahoma Medical Research Foundation, Oklahoma City.;Corvallis Environmental Research Lab., OR. |
| Publisher |
c1988 |
| Year Published |
1988 |
| Report Number |
EPA-R-812710; EPA/600/J-88/225; |
| Stock Number |
PB89-144562 |
| Additional Subjects |
Deoxyribonucleic acids ;
Free radicals ;
Guanines ;
Ultraviolet radiation ;
Hydrogen peroxide ;
Ethyl alcohol ;
Thioureas ;
Salicylates ;
Glutathione ;
Thymus gland ;
Sugar alcohols ;
Scavenging ;
Reprints ;
Hydroxyguanine ;
Biological radiation effects ;
Hydroxyl radicals ;
DNA ;
Cows ;
Mannitol
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB89-144562 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
9p |
| Abstract |
Formation of 8-hydroxyguanine within calf thymus DNA has been studied after exposure to uv-H2O2 as a hydroxyl free radical generating system. Using high-pressure liquid chromatography with electrochemical detection, the amount of 8-hydroxy-2-deoxyguanosine (8-OHdG) was measured. The 8-OHdG content of uv-exposed DNA increased linearly with increasing H2O2 levels up to 0.03%, above which the rate of increase was less than linear. All hydroxyl free radical scavengers studied (mannitol, ethanol, thiorea, and salicylate), if present in the system when DNA was exposed to uv-H2O2, caused a decrease in the amount of 8-OHdG formed. Thiourea when incubated with damaged DNA caused a loss of 8-OHdG when it was an integral part of DNA. In contrast, thiorea did not react with the nucleoside free in solution. Reduced glutathione did not cause a decrease of 8-OHdG, either when it was an integral part of DNA, or as the free nucleoside in solution. (Copyright (c) 1988 by Academic Press, Inc.) |
| Supplementary Notes |
Pub. in Archives of Biochemistry and Biophysics, v262 n1 p266-272 1988. Sponsored by Corvallis Environmental Research Lab., OR. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Hydroxyl Free Radical Mediated Formation of 8-Hydroxyguanine in Isolated DNA (Journal Version). |
| Category Codes |
57F; 57V; 57B |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/02 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
908927736 |
| Origin |
NTIS |
| Type |
CAT |
