| Main Title |
Transfer of Arsenite from Glutathione to Dithiols: A Model of Interaction. |
| Author |
Delnomdedieu, M. ;
Basti, M. M. ;
Otvos, J. D. ;
Thomas, D. J. ;
|
| CORP Author |
North Carolina Univ. at Chapel Hill. Center for Environmental Medicine and Lung Biology. ;North Carolina State Univ. at Raleigh. Dept. of Biochemistry.;Health Effects Research Lab., Research Triangle Park, NC. Pharmacokinetics Branch. |
| Publisher |
cOct 93 |
| Year Published |
1993 |
| Report Number |
EPA-R-817643; EPA/600/J-94/532; |
| Stock Number |
PB95-148946 |
| Additional Subjects |
Glutathione ;
Arsenites ;
Chemical models ;
Thiols ;
Nuclear magnetic resonance spectroscopy ;
Binding sites ;
Arsenates ;
Reduction(Chemistry) ;
Carbon 13 ;
Reprints ;
Succinic acid/meso-2,3-dimercapto
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB95-148946 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
6p |
| Abstract |
The interactions of arsenate and arsenite with meso-2,3-dimercaptosuccinic acid (DMSA) have been characterized using carbon-13 nuclear magnetic resonance. These studies show that DMSA reduces arsenate to arsenite and complexes arsenite. Monitoring the carbon-13 signals of complexed DMSA and liberated glutathione shows that DMSA readily extracts arsenite from a glutathione(sub 3)-arsenite complex, proving the affinity of arsenite for dithiols is greater than that for monothiols. Competition between DMSA (vicinal thiols) and dithioerythritol (1,4-dimercapto-2,3-butanediol) for binding of arsenite indicates that the binding affinity is inversely related to the distance between the two thiol groups. On the basis of these findings, a model for the interaction of arsenic with mono- and dithiol-containing molecules is proposed. (Copyright (c) 1993 by the American Chemical Society.) |
| Supplementary Notes |
Pub. in Chemical Research in Toxicology 6, n5 p598-602 Oct 93. Prepared in cooperation with North Carolina State Univ. at Raleigh. Dept. of Biochemistry. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. Pharmacokinetics Branch. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Transfer of Arsenite from Glutathione to Dithiols: A Model of Interaction. |
| Category Codes |
99D |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
503415431 |
| Origin |
NTIS |
| Type |
CAT |
