| Main Title |
Effects of 2,4-Dichlorophenol, a Metabolite of a Genetically Engineered Bacterium, and 2,4-Dichlorophenoxyacetate on Some Microorganism-Mediated Ecological Processes in Soil. |
| Author |
Short, K. A. ;
Doyle, J. D. ;
King, R. J. ;
Seidler, R. J. ;
Stotzky, G. ;
|
| CORP Author |
Corvallis Environmental Research Lab., OR. ;NSI Technology Services Corp., Corvallis, OR. Environmental Research Lab. ;New York Univ., NY. Dept. of Biology. ;Michigan Univ., Ann Arbor. Medical School. |
| Publisher |
c1991 |
| Year Published |
1991 |
| Report Number |
EPA/600/J-91/144; |
| Stock Number |
PB91-219022 |
| Additional Subjects |
Genetic engineering ;
Pseudomonas ;
Soil microbiology ;
Terrestrial ecosystems ;
Pesticides ;
Soil contamination ;
Mass fragmentography ;
Metabolism ;
Glucose ;
Plasmids ;
Fungi ;
Toxicity ;
Kinetics ;
Reprints ;
Dichlorophenols ;
Dichlorophenoxyacetates
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB91-219022 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
9p |
| Abstract |
A genetically engineered microorganism, Pseudomonas putida PP0301 (pR0103), and the plasmidless parent strain, PP0301, were added at approximately ten to the seventh CFU/g of soil amended with 500 ppm of 2,4-dichlorophenoxyacete (2,4-D) (500 microgram/g). The degradation of 2,4-D and the accumulation of a single metabolite, identified by gas chromatography-mass spectrophotometry as 2,4-dichlorophenol (2,4-DCP), occurred only in soil inoculated with PP0301 (pR0103), wherein 2,4-DCP accumulated to >70 ppm for 5 weeks and the concentration of 2,4-D was reduced to <100 ppm. Coincident with the accumulation of 2,4-DCP was a >400-fold decline in the numbers of fungal propagules and a marked reduction in the rate of CO2 evolution, whereas 2,4-D did not depress either fungal propagules or respiration of the soil microbiota. 2,4-DCP did not appear to depress the numbers of total heterotrophic, sporeforming, or chitin-utilizing bacteria. (Copyright (c) 1991, American Society for Microbiology.) |
| Supplementary Notes |
Pub. in Applied and Environmental Microbiology, v57 n2 p412-418 Feb 91. Prepared in cooperation with NSI Technology Services Corp., Corvallis, OR. Environmental Research Lab., New York Univ., NY. Dept. of Biology, and Michigan Univ., Ann Arbor. Medical School. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Effects of 2,4-Dichlorophenol, a Metabolite of a Genetically Engineered Bacterium, and 2,4-Dichlorophenoxyacetate on Some Microorganism-Mediated Ecological Processes in Soil. |
| Category Codes |
68E; 57K; 57F; 57B |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/02 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
126923588 |
| Origin |
NTIS |
| Type |
CAT |
