| Main Title |
Recovery of Bulk DNA from Soil by a Rapid, Small-Scale Extraction Method. |
| Author |
Porteous, L. A. ;
Armstrong, J. L. ;
|
| CORP Author |
Corvallis Environmental Research Lab., OR. |
| Publisher |
c1991 |
| Year Published |
1991 |
| Report Number |
EPA/600/J-91/225; |
| Stock Number |
PB92-108141 |
| Additional Subjects |
Extraction ;
Soils ;
Deoxyribonucleic acids ;
Separation ;
Humic acids ;
Centrifuging ;
Ecology ;
Reprints ;
Lysozyme ;
Novogym
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB92-108141 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
6p |
| Abstract |
The authors describe an extraction method that yields restrictable 20-25 kb DNA from one gram of soil. Cells are lysed directly in the soil. The crude DNA extract is separated from contaminating humic compounds, concentrated, and purified by CsCl gradient centrifugation and the commercial product Geneclean. |
| Supplementary Notes |
Pub. in Current Microbiology, v22 p345-348 1991. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Recovery of Bulk DNA from Soil by a Rapid, Small-Scale Extraction Method. |
| Category Codes |
57K; 57H |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/02 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
133919624 |
| Origin |
NTIS |
| Type |
CAT |
