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Main Title Rapid Glutamate Decarboxylase Assay for Detection of 'Escherichia coli'.
Author Rice, E. W. ; Johnson, C. H. ; Dunnigan, M. E. ; Reasoner, D. J. ;
CORP Author Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. ;Cincinnati Univ., OH. Coll. of Medicine.
Publisher 1993
Year Published 1993
Report Number EPA/600/J-93/496;
Stock Number PB94-130143
Additional Subjects Escherichia coli ; Test methods ; Bioassay ; Glutamates ; Drinking water ; Enzymes ; Decarboxylation ; Culture media ; Sampling ; Cells(Biology) ; Exposure ; Chlorine ; Glutamate decarboxylase
Holdings
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NTIS  PB94-130143 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 5p
Abstract A rapid test procedure for the enzyme glutamate decarboxylase was developed for the detection of Escherichia coli. The assay procedure was able to confirm the presence of E. coli in enteric broth cultures with a 95 percent specificity for both pure cultures and environmental samples. The procedure was capable of detecting survivors of chlorine exposed cells and was comparable to EC-MUG medium in the multiple tube fermentation procedure.
Supplementary Notes Pub. in Applied and Environmental Microbiology, v59 n12 p4347-4349 Dec 93. Prepared in cooperation with Cincinnati Univ., OH. Coll. of Medicine.
NTIS Title Notes Journal article.
Category Codes 48B; 57K; 57F; 68D
NTIS Prices PC A01/MF A01
Primary Description 600/14
Document Type NT
Cataloging Source NTIS/MT
Control Number 404817043
Origin NTIS
Type CAT