||Improved Flow Cytometric Assay for Somatic Mutations at the Glycophorin A Locus in Humans.
Langlois, R. G. ;
Nisbet, B. A. ;
Bigbee, W. L. ;
Ridinger, D. N. ;
Jensen., R. H. ;
||Lawrence Livermore National Lab., CA. Biomedical Sciences Div. ;Utah Univ., Salt Lake City. Dept. of Internal Medicine.;Health Effects Research Lab., Research Triangle Park, NC.;Department of Energy, Washington, DC.;National Institutes of Health, Bethesda, MD.
Clinical chemistry ;
Ionizing radiation ;
Chromosome mapping ;
Flow cytometry ;
Fluorescent antibody technic ;
||Some EPA libraries have a fiche copy filed under the call number shown.
||An improved method has been developed for the glycophorin A assay for somatic cell mutations in humans. The new assay, named the 'BR6' assay, can be performed on a commercially available, single-beam flow cytometer, in contrast to the previously described 1W1 assay that required a dual-beam flow sorter. A modified cell labeling method developed for the BR6 assay results in improved separation of normal and mutant phenotype cells, as well as eliminating some cellular artifacts that affected the 1W1 assay. Parallel measurements on samples from 17 normal donors showed that the BR6 assay yields comparable variant cell frequencies and improved measurement precision compared with the 1W1 assay. (Copyright (c) 1990 Wiley-Liss Inc.)
||Pub. in Cytometry, v11 p513-521 Apr 90. Prepared in cooperation with Utah Univ., Salt Lake City. Dept. of Internal Medicine. Sponsored by Health Effects Research Lab., Research Triangle Park, NC., Department of Energy, Washington, DC., and National Institutes of Health, Bethesda, MD.
|NTIS Title Notes
||Reprint: Improved Flow Cytometric Assay for Somatic Mutations at the Glycophorin A Locus in Humans.
||PC A03/MF A01