| Main Title |
Changes in Accessibility of DNA to Various Fluorochromes during Spermatogenesis. |
| Author |
Evenson, D. ;
Darzynkiewicz, Z. ;
Jost, L. ;
Janca, F. ;
Ballachey, B. ;
|
| CORP Author |
South Dakota State Univ., Brookings. Dept. of Chemistry. ;Sloan-Kettering Inst. for Cancer Research, New York. Lab. of Investigative Cytology.;Health Effects Research Lab., Research Triangle Park, NC. |
| Year Published |
1986 |
| Report Number |
EPA/600/J-86/163; |
| Stock Number |
PB87-114609 |
| Additional Subjects |
Deoxyribonucleic acid ;
Flow cytometry ;
Reprints ;
Fluorochromes ;
Spermatogenesis
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB87-114609 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
11p |
| Abstract |
Accessibility of mouse testicular and vas deferens (vas) sperm cell DNA to acridine orange, propidium iodide, ellipticine, Hoechst 33342, mithramycin, chromomycin A3, 4'6-diamidino-2-phenylindole (DAPI), and 7-amino-actinomycin D (7-amino-AMD) was determined by flow cytometry. Permealized cells were either stained directly or after pretreatment with 0.06 N HCI. For histone-containing tetraploid, diploid, and round spermatid cells, HCI extraction of nuclear proteins caused an approximately sixfold increase of 7-amino-AMD stainability but had no significant effect on DAPI stainability. For these same cell types, the stainability with other intercalating (acridine orange, propidium iodide, ellipticine) and externally binding (Hoechst 33342, mithramycin, chromomycin A3) dyes was increased by 1.6- to 4.0-fold after HCI treatment. In sharp contrast, HCI treatment of vas sperm did not increase the staining level of 7-amino-AMD, DAPI, or propidium iodide but did increase the staining level for the other intercalating dyes (1.3- to 1.5-fold) and external dyes (1.3- to 1.9-fold). |
| Supplementary Notes |
Pub. in Cytometry 7, p45-53 Jan 86. Prepared in cooperation with Sloan-Kettering Inst. for Cancer Research, New York. Lab. of Investigative Cytology. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Changes in Accessibility of DNA to Various Fluorochromes during Spermatogenesis. |
| PUB Date Free Form |
c1986 |
| Category Codes |
57F |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
703715103 |
| Origin |
NTIS |
| Type |
CAT |
