Main Title |
Use of a Novel Plasmid to Monitor the Fate of a Genetically Engineered 'Pseudomonas putida' Strain. |
Author |
Genthner, F. J. ;
Campbell, R. P. ;
Pritchard, P. H. ;
|
CORP Author |
Environmental Research Lab., Gulf Breeze, FL. ;Technical Resources, Inc., Gulf Breeze, FL. |
Publisher |
c1992 |
Year Published |
1992 |
Report Number |
EPA/600/J-93/066; |
Stock Number |
PB93-169001 |
Additional Subjects |
Pseudomonas putida ;
Genetic engineering ;
Antibiotic resistance ;
Plasmids ;
Deoxyribonucleic acids ;
Nucleic acid hybridization ;
Aquatic microbiology ;
Biodeterioration ;
Phenotype ;
Growth ;
Reprints ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB93-169001 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
9p |
Abstract |
Plasmid pSI30 was constructed to increase the sensitivity of detection of a genetically engineered microorganism (GEM) and its recombinant DNA in environmental samples. The broad host-range, mobilizable plasmid contained chlorocatechol (clc) degradative genes, antibiotic resistance genes (ampicillin and kanamycin), and a fragment of eukaryotic DNA. The clc genes encode enzymes that convert 3-chlorocatechol to maleylacetic acid permitting the host, Pseudomonas putida RC-4, to grow on 3-chlorobenzoate. The catabolic phenotype was exploited using enrichment procedures to detect RC-4(pSI30) cells, freeliving in the water column or when irreversibly bound to surfaces. The eukaryotic DNA sequence provided a unique target allowing positive identification by DNA:DNA hybridization. In flow-through microcosms, RC-4(pSI30), undetectable as freeliving cells, was found by enrichment as irreversibly bound sessile forms. These experiments revealed the stability of pSI30 and its utility in a combination detection system for tracking the survival of a GEM and its DNA in environmental samples. |
Supplementary Notes |
Pub. in Molecular Ecology 1, p137-143 1992. See also PB91-206870. Prepared in cooperation with Technical Resources, Inc., Gulf Breeze, FL. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Use of a Novel Plasmid to Monitor the Fate of a Genetically Engineered 'Pseudomonas putida' Strain. |
Category Codes |
57K; 57F |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/04 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
314124355 |
Origin |
NTIS |
Type |
CAT |