| Main Title |
Relationships between Benzo(a)pyrene-DNA Adduct Levels and Genotoxic Effects in Mammalian Cells. |
| Author |
Arce, G. T. ;
Allen, J. W. ;
Doerr, C. L. ;
Elmore, E. ;
Hatch, G. G. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Northrop Services, Inc./Environmental Sciences, Research Triangle Park, NC. ;Columbia Univ., New York. Coll. of Physicians and Surgeons. |
| Year Published |
1987 |
| Report Number |
EPA/600/J-87/172; |
| Stock Number |
PB88-160668 |
| Additional Subjects |
Deoxyribonucleic acid ;
Mammals ;
Cells(Biology) ;
Reprints ;
Benzo(a)pyrene ;
DNA-adduct
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB88-160668 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
10p |
| Abstract |
A modification of the doubling dose concept taken from studies in radiation biology was applied to DNA-adduct and genetic toxicology data. The modification, the doubling adduct level, is defined as the number of DNA adducts per unit of DNA required to double the induced frequency of genotoxic response. The data was obtained from concurrent studies, measuring benzo(a)pyrene (B(a)P) induced genotoxic effects and DNA adducts in several short-term bioassay systems: cytotoxicity, gene mutation, and sister-chromatid-exchange (SCE) in Chinese hamster V79 cells; cytotoxicity, gene mutation, and chromosome aberrations in mouse lymphoma L5178Y TK+/- cells; cytotoxicity and enhanced virus transformation in Syrian hamster embryo cells; and cytotoxicity and morphological transformation in C3H10T1/2CL8 mouse embryo fibroblasts. Both total B(a)P-DNA binding and specific B(a)P-DNA adducts were measured. N2(10beta(7beta, 8alpha,9alpha-trihydroxy-7,8,9,10-tetra-hydrobenzo(a)pyrene)ly)deoxyguanosine (BPDE I-dG) was one of the major adducts identified in all bioassay systems. |
| Supplementary Notes |
Pub. in Cancer Research, v47 p3388-3395, 1 Jul 87. Prepared in cooperation with Northrop Services, Inc./Environmental Sciences, Research Triangle Park, NC., and Columbia Univ., New York. Coll. of Physicians and Surgeons. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Relationships between Benzo(a)pyrene-DNA Adduct Levels and Genotoxic Effects in Mammalian Cells. |
| PUB Date Free Form |
c1987 |
| Category Codes |
57B |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
809821010 |
| Origin |
NTIS |
| Type |
CAT |
