| Main Title |
Development of an Intact Hepatocyte Activation System for Routine Use with the Mouse Lymphoma Assay. |
| Author |
Brock, K. H. ;
Moore, M. M. ;
Oglesby, L. A. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Northrop Services, Inc., Research Triangle Park, NC. |
| Year Published |
1987 |
| Report Number |
EPA/600/J-87/194; |
| Stock Number |
PB88-165998 |
| Additional Subjects |
Hepatocytes ;
Lymphoma ;
In viro analysis ;
Mice ;
Rats ;
Cell cultures ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB88-165998 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
13p |
| Abstract |
The authors have developed a method for cocultivating primary rat hepatocytes with L5178Y/TK+/- 3.7.2C mouse lymphoma cells. The system should provide a means to simulate more closely in vivo metabolism compared to metabolism by liver homogenates, while still being useful for routine screening. Hepatocytes were isolated from 200-250 g adult male Sprague-Dawley rats. Rapid attachment of the hepatocytes (2 h) was obtained by using fibronectin-coated 25-cm2 tissue culture flasks. Several factors proved important in obtaining optimal cocultivated mouse lymphoma cell growth. These included maintaining the cocultivated cultures on a rocker platform instead of stationary and either (1) reducing the cocultivation time from 16 to 4 h or (2) utilizing less than 1 x 10 to the 6th power hepatocytes per flask for 16-h exposures. CP, DMN, DMBA, and B(a)P were used to demonstrate the capability of the system to detect mutagens that require metabolic activation. |
| Supplementary Notes |
Pub. in Environmental Mutagenesis, v9 p331-341 1987. Prepared in cooperation with Environmental Health Research and Testing, Inc., Research Triangle Park, NC., and Northrop Services, Inc., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Development of an Intact Hepatocyte Activation System for Routine Use with the Mouse Lymphoma Assay. |
| PUB Date Free Form |
c1987 |
| Category Codes |
57F |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
811323901 |
| Origin |
NTIS |
| Type |
CAT |
