Main Title |
Application of a Wheat Seedling Assay for Detecting Aneuploidy Induced by N-Ethyl-N-Nitrosourea and 4-Nitroquinoline-1-Oxide. |
Author |
Dhesi, J. S. ;
Sandhu, S. S. ;
|
CORP Author |
North Carolina Central Univ., Durham. Dept. of Biology.;Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. |
Publisher |
c1992 |
Year Published |
1992 |
Report Number |
EPA-68-01-4456; EPA/600/J-92/447; |
Stock Number |
PB93-141463 |
Additional Subjects |
Wheat ;
Aneuploidy ;
Ethylnitrosourea ;
4-Nitroquinoline-1-oxide ;
Seeds ;
Toxicity ;
Chromosomes ;
Alleles ;
Risk assessment ;
Temperature ;
Dose-response relationships ;
Reprints ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB93-141463 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
9p |
Abstract |
N-ethyl-N-nitrosourea and 4-nitroquinoline-1-oxide were evaluated in the allohexapolyploid wheat seedling assay for their ability to induce aneuploidy and/or small chromosome deletions. The wheat strain used (Neatby's virescent) is homozygous for a pair of recessive alleles (v1) present on chromosome 3B and produces virescent seedlings when grown at temperatures below 26 C. When the germinating embryos are treated with a test chemical, loss of chromosome 3B or its segment bearing the v1 allele produces a green sector in the leaf whereas a gain of this chromosome induces a white sector. Both the test chemicals yielded positive results in this assay. The assay is very simple and can be employed for evaluating the genetic potential of chemicals in a laboratory as well as for in situ hazards assessment under natural environmental conditions. |