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RECORD NUMBER: 4 OF 66

OLS Field Name OLS Field Data
Main Title Application of a Wheat Seedling Assay for Detecting Aneuploidy Induced by N-Ethyl-N-Nitrosourea and 4-Nitroquinoline-1-Oxide.
Author Dhesi, J. S. ; Sandhu, S. S. ;
CORP Author North Carolina Central Univ., Durham. Dept. of Biology.;Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.
Publisher c1992
Year Published 1992
Report Number EPA-68-01-4456; EPA/600/J-92/447;
Stock Number PB93-141463
Additional Subjects Wheat ; Aneuploidy ; Ethylnitrosourea ; 4-Nitroquinoline-1-oxide ; Seeds ; Toxicity ; Chromosomes ; Alleles ; Risk assessment ; Temperature ; Dose-response relationships ; Reprints ;
Holdings
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Status
NTIS  PB93-141463 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/12/1993
Collation 9p
Abstract
N-ethyl-N-nitrosourea and 4-nitroquinoline-1-oxide were evaluated in the allohexapolyploid wheat seedling assay for their ability to induce aneuploidy and/or small chromosome deletions. The wheat strain used (Neatby's virescent) is homozygous for a pair of recessive alleles (v1) present on chromosome 3B and produces virescent seedlings when grown at temperatures below 26 C. When the germinating embryos are treated with a test chemical, loss of chromosome 3B or its segment bearing the v1 allele produces a green sector in the leaf whereas a gain of this chromosome induces a white sector. Both the test chemicals yielded positive results in this assay. The assay is very simple and can be employed for evaluating the genetic potential of chemicals in a laboratory as well as for in situ hazards assessment under natural environmental conditions.