Main Title |
Simplified Radioimmunoassay for Detection of Human Rotavirus in Stools. |
Author |
Cukor, George ;
Berry, Mary Kay ;
Blacklow, Neil R. ;
|
CORP Author |
Massachusetts Univ. Medical School, Worcester.;Health Effects Research Lab., Cincinnati, OH. |
Year Published |
1981 |
Report Number |
EPA-R-805169 ;EPA-R-806591-01-0; EPA/600/J-78/184; |
Stock Number |
PB81-231110 |
Additional Subjects |
Diagnosis ;
Humans ;
Infants ;
Electron microscopy ;
Reprints ;
Radioimmunoassay ;
Rotaviruses
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB81-231110 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
7p |
Abstract |
A simplified radioimmunoassay (RIA) technique was developed to facilitate the diagnosis of human rotavirus in stools of infants with diarrhea. This microtiter solid-phase RIA utilizes as a critical reagent hyperimmune serum against a tissue culture-grown simian rotavirus that is immunologically closely related to the human agent. One hundred thirteen pediatric stool samples were tested. Thirty-five specimens known to be positive for rotavirus by electron microscopy were also positive by RIA. All RIA-positive reactions (36) were shown to be specific for rotavirus by means of an antibody-blocking test. Nonspecific RIA-positive reactions were not encountered. This accurate, sensitive RIA technique is practical for use in diagnostic laboratories because critical serum reagents are prepared against culture-grown virus and not against rotavirus derived from carefully selected stools of humans or experimentally infected gnotobiotic animals. |