Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Bethnic Bioassay Using Time-Lapse Photography to Measure the Effect of Toxicants on the Feeding Behavior of Lugworms (Polychaeta:Arenicolidae).
Author Rubinstein, Norman I. ;
CORP Author University of West Florida, Pensacola. Dept. of Biology.;Environmental Protection Agency, Gulf Breeze, FL. Gulf Breeze Environmental Research Lab.
Year Published 1979
Report Number EPA-R-804458; EPA-600/J-79-097;
Stock Number PB80-197833
Additional Subjects Toxicology ; Pesticides ; Bioassay ; Measurement ; Photography ; Ingestion(Biology) ; Exposure ; Activity ; Chlorine organic compounds ; Concentration(Composition) ; Reprints ; Toxic substances ; Arenicola cristata ; Lugworms ; Kepone
Library Call Number Additional Info Location Last
NTIS  PB80-197833 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 13p
A benthic assay was developed utilizing time-lapse photography to measure the feeding activity of a lugworm, Arenicola cristata. Automated 35 mm cameras were used to record formation of feeding funnels at 12-hour intervals. Substrate surface area reworked by lugworms held under identical conditions in separate aquaria was plotted against time to determine substrate reworking rates for each group. Rates were subjected to linear regression analysis and compared to demonstrate that no significant difference between the slopes of the calculated lines existed. Therefore, a difference in slope when one group is exposed to a toxicant could provide a measure of effect on lugworm activity. Lugworms also were exposed to the pesticide, Kepone, and their rate of substrate reworking was compared with unexposed lugworms. Kepone was acutely toxic to lugworms at a concentration of 29.5 micrograms/l. A significant difference in substrate reworking rates was observed following exposure to concentrations as low as 2.8 micrograms/l Kepone in seawater. It is suggested that a behavioral response to toxicity testing provides a sensitive and realistic approach for evaluation of ecological impact of pollutants on the marine environment.