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RECORD NUMBER: 17 OF 26

OLS Field Name OLS Field Data
Main Title Importance of Glycolysable Substrates for In vitro Capacitation of Human Spermatozoa.
Author Rogers, B. J. ; Perreault, S. D. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Reproductive Toxicology Branch. ;Vanderbilt Univ., Nashville, TN. School of Medicine.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/358;
Stock Number PB91-163725
Additional Subjects Culture media ; Spermatozoa ; Glycolysis ; Humans ; Sperm-ovum interactions ; Sperm motility ; Sugars ; In vitro analysis ; Dose-response relationships ; Zona pellucida ; Reprints ;
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB91-163725 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/13/1991
Collation 8p
Abstract
To investigate the importance of glycolysable substrate for supporting the ability of human sperm to capacitate and penetrate oocytes in vitro, washed spermatozoa were incubated with or without various sugars in BWW culture medium containing pyruvate and lactate. Sperm penetration was assayed using zona-free hamster oocytes. After an 18-h preincubation, glucose (1 mg/ml) supported higher penetration of sperm into oocytes than either mannose or fructose (60.7% vs 28.2% or 21.5%, respectively) at the same concentration. Penetration was even lower when medium contained the nonmetabolizable sugar galactose (2.1% at 1 mg/ml). On the other hand, higher concentrations (5 or 10 mg/ml) of glucose, but not fructose, suppressed penetration, provided the glucose was present throughout the 18-h preincubation. When caffeine, a stimulant of glycolysis in human sperm, was present along with glucose, sperm penetration was enhanced, but only after 6 h of sperm preincubation. The effect was not observed in glucose-free medium, however, where penetration remained low over a 10-h incubation period. In these experiments, the percentage of motile sperm was unaffected by treatment, but the quality of motility was diminished in the absence of glucose. Stimulation of glycolysis may promote capacitation of human spermatozoa in vitro and that optimization of penetrating ability of sperm is dependent upon both the type and concentration of glycolysable sugar present.