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RECORD NUMBER: 32 OF 287

OLS Field Name OLS Field Data
Main Title Characterization of Catalase Activities in a Root-Colonizing Isolate of 'Pseudomonas putida' (Revised).
Author Katsuwon, J. ; Anderson, A. J. ;
CORP Author Utah Agricultural Experiment Station, Logan.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1991
Year Published 1991
Report Number PAPER-4175; EPA/600/J-93/222;
Stock Number PB93-205052
Additional Subjects Catalase ; Pseudomonas putida ; Plants(Botany) ; Microbial colony count ; Isoenzymes ; pH ; Hydrogen peroxide ; Peroxidases ; Mutations ; Reprints ;
Holdings
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Status
NTIS  PB93-205052 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 11/22/1993
Collation 9p
Abstract
Pseudomonas putida, a saprophytic root-colonizing bacterium, produces multiple forms of catalase. Catalase A, which increases in specific activity during growth phase and after treatment with H2O2, is located in the cytoplasm and is inhibited by 3-amino-1,2,4-triazole, EDTA, and cyanide, but not by chloroform-methanol treatment. Catalase B, which is induced by external H2O2 or during stationary phase of growth, is membrane associated and is inhibited by chloroform-methanol, EDTA, and cyanide, but not by aminotriazole. Catalase A has a broad pH optimum, from pH 6.0 to 11.0, with two peaks, at pH 8.0 and 11.0. Catalase B is most active at pH 5.0-11.0 Mutant J-1, generated by ethyl methanesulfonate mutagenesis, lacked catalase A activty in extracts of cells harvested throughout lag to early stationary growth phase in liquid medium. Catalase B was produced by J-1 in stationary phase. Exposure of J-1 to H2O2 caused the production of both catalase A and catalase B. Mutant J-1 was more susceptible to cell death than the wild type upon direct exposure to 2.5 mM H2O2 but survived the treatment after exposure to lower (0.3 mM), nonlethal doses of H2O2. The ability to adapt to H2O2 may be related to the behavior of J-1 on roots where active oxygen species are produced by root surface enzymes. J-1 colonized root surfaces at wild-type levels and produced catalases A and B after exposure to root surfaces for 12 h.