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RECORD NUMBER: 218 OF 281

OLS Field Name OLS Field Data
Main Title Physical and Chemical Parameters of Sediment Extraction and Fractionation That Influence Toxicity, as Evaluated by Microtox (Trade Name).
Author Ho, K. T. Y. ; Quinn, J. G. ;
CORP Author Rhode Island Univ., Narragansett. Graduate School of Oceanography.;Environmental Research Lab., Narragansett, RI.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-93/141 ; ERLN-1447
Stock Number PB93-185882
Additional Subjects Organic compounds ; Sediments ; Toxicity ; Bioassay ; Hazardous materials ; Estuaries ; Chemical analysis ; Fractionation ; Extraction ; Acetone ; Methanol ; Acetonitrile ; Reprints ;
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB93-185882 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 08/23/1993
Collation 13p
Abstract
Several physical and chemical parameters of sediment extraction and fractionation of organic compounds that influence bioassay results were evaluated. Each parameter was evaluated with a photoluminescent bacterial bioassay (Microtox) as an end point. Three solvents (acetonitrile, acetone, and methanol) were studied for their ability to extract toxic organic components from marine sediments. Acetone extracted the most toxicity, with no difference between acetonitrile and methanol. Two methods of fractionating sediment extracts (silica-gel-column chromatography (SGCC) and acid-base fractionation) were compared. SGCC was more useful because it resulted in a wider range of responses and was faster to perform than acid-base fractionation. Microtox was used to rank four marine sediments with respect to toxicity and to determine if one chemical class (or fraction) was consistently more toxic among different sediments. With some caveats, Microtox results agreed with general chemical concentration trends and other bioassay results in distinguishing between contaminated and noncontaminated sediments. Although results indicated there was not a consistently most toxic fraction among sediments, there was a consistently least toxic fraction. The effect of sediment storage time on toxicity was also evaluated. Results indicated that the most stable chemical fraction (containing nonpolar hydrocarbons) did not change toxicologically for 30 weeks, whereas the more chemically active fraction (containing ketones, quinones, and carboxyls) changed as soon as one week. (Copyright (c) 1993 SETAC.)