Record Display for the EPA National Library Catalog

RECORD NUMBER: 48 OF 215

OLS Field Name OLS Field Data
Main Title Effects of Holding Time, Storage, and the Preservation of Samples on Sample Integrity for the Detection of Fecal Indicator Bacteria by Quantitative Polymerase Chain Reaction (QPCR)-based Assays.
Author L. Wymer ; K. Oshima ; J. Paar ; M. Doolittle ; J. Lavender
Other Authors
Author Title of a Work
M. Varma
R. Haugland
CORP Author TechLaw, Inc., Bethesda, MD.; Environmental Protection Agency, Lexington, MA. Region 1.
Year Published 2010
Report Number EPA/600/R-10/150
Stock Number PB2013-107494
Additional Subjects Bacteria ; Water pollution monitoring ; Fecal pollution ; Reagents ; Membrane filters ; Standards ; Equipment ; Supplies ; Safety ; Sampling ; Quality control ; Calibration ; Enteric pathogens ; Pollution prevention ; Waste management ; RNA gene sequences ; Quantitative polymerase chain reaction (qPCR) ; TaqMan ; Enterococci
Internet Access
Description Access URL
https://nepis.epa.gov/Exe/ZyPDF.cgi?Dockey=P100NG0Z.PDF
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB2013-107494 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 01/17/2014
Collation 58p
Abstract
The purpose of this project was to answer questions related to storage of samples to be analyzed by the quantitative polymerase chain reaction (qPCR)-based assays for fecal indicator bacteria. The report is divided into two parts. The first part describes studies that were performed to determine if filters that are used to collect fecal indicator bacteria can be stored frozen and analyzed at a later date. These studies were primarily directed at a specific, targeted question: can qPCR results from freezer archived samples be used to establish valid relationships between fecal indicator densities and health effects data collected from previous epidemiological studies. The second part describes studies that were performed to determine if refrigerated water samples can be held for 24 to 48 hours prior to analysis by qPCR. These studies addressed a question that may be relevant to the implementation of the qPCR method for water quality monitoring at remote locations where immediate analysis of the samples is not possible.