Record Display for the EPA National Library Catalog
RECORD NUMBER: 27 OF 38Main Title | Method 1603 : Escherichia coli (E. coli) in water by membrane filtration using modified membrane-thermotolerant Escherichia coli agar (modified mTEC). | |||||||||||
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CORP Author | Environmental Protection Agency, Washington, DC. Office of Water. | |||||||||||
Publisher | United States, Environmental Protection Agency, Office of Water, | |||||||||||
Year Published | 2002 | |||||||||||
Report Number | EPA/821/R-02/023 | |||||||||||
Stock Number | PB2003-100128 | |||||||||||
Subjects | Escherichia coli--United States--Prevention | |||||||||||
Additional Subjects | Escherichia coli ; Membrane filtration ; Water sampling ; Bacteria ; Agar ; Epidemiology ; Water pollution effects ; Marine environment ; Fresh water ; Health effects ; Gastrointestines ; Feces ; Substrates ; Surface waters ; Water quality ; Counting methods ; Calibration ; Contamination ; Laboratories ; Test methods | |||||||||||
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Abstract | The method describes a revised Escherichia coli membrane filter (MF) procedure, a single-step method that uses one medium, modified mTEC Agar, and does not require the transfer of the membrane filter to another medium or other substrate. The modified medium contains a chromogen (5-bromo-6-chloro- 3-indolyl--D-glucuronide), which is catabolized to glucuronic acid and a red- or magenta-colored compound byE. coli that produce the enzyme D-glucuronidase. The apparatus and equipment, and sampling, filtration, and verification procedures for this modified mTEC method are identical to those of the original mTEC method. Because the bacterium is a natural inhabitant only of the intestinal tract of warm-blooded animals, its presence in water samples is an indication of fecal pollution and the possible presence of entericpathogens. |
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Notes | "September 2002." "EPA-821-R-02-023." Includes bibliographical references. Microfiche. |