||DNA Isolation from Small Tissue Samples Using Salt and Spermine.
Ross, J. A. ;
Nelson, G. B. ;
Holden, K. L. ;
||Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Deoxyribonucleic acids ;
Precipitin tests ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
Common DNA isolation methods rely upon protein denaturation by organic solvents such as phenol and chloroform. These solvents pose some risk to the user and require special disposal procedures. The authors have previously reported a method for isolating DNA from peripheral blood lymphocytes by precipitation with spermine. This procedure was less effective with other tissues, especially lung, and the spermine precipitates often took several days to dissolve in buffer. The authors have modified their procedure to include a pre-extraction of proteins with concentrated NaCl, essentially as described by Miller et al. This current, improved procedure provides good recovery of purified DNA even with small samples (< 200 mg) and a variety of tissue and cell types.