Main Title |
Non-radioactive labeling and detection of biomolecules |
Other Authors |
|
Publisher |
Springer-Verlag, |
Year Published |
1992 |
OCLC Number |
26362206 |
ISBN |
3540554823 (alk. paper) : 0387554823 (alk. paper : U.S.) |
Subjects |
Biomolecules--Labeling ;
Biological Markers
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
ELBM |
QP519.9.A37N65 1992 |
|
AWBERC Library/Cincinnati,OH |
05/13/1994 |
|
Collation |
xxiii, 436 p. |
Notes |
Includes bibliographical references and index. |
Contents Notes |
General aspects of nonradioactive labeling and detection -- Standard nonradioactive labeling and detection systems -- Overview of nonradioactive labeling systems -- Digoxigenin: anti-digoxigenin (DIG) system -- Biotin system -- In vivo labeling of DNA probes with 5-BrdU -- Sulfone system -- Colloidal gold as a marker in molecular biology: the use of ultra-small gold probes -- Direct peroxidase labeling of hybridization probes and chemiluminescence detection -- Highly sensitive method for detecting peroxidase in in situ hybridization or immunohistochemical assays -- SNAP system -- Specialized nonradioactive detection systems -- Overview of colorimetric, chemiluminometric, and fluorimetric detection systems -- Colorimetric systems -- Luminescence systems -- Fluorescence systems -- Enhanced systems -- Overview of amplification systems -- Enhanced signal generation by target amplification -- Signal amplification system: substrate cascade -- Application formats -- Overview of application formats -- Blot formats: nucleic acids -- Blot formats: proteins and glycoproteins. In situ formats -- Quantitative formats -- Suppliers of reagents -- Suppliers of equipment. |