Main Title |
A tissue enzyme assay for chlorinated hydrocarbon insecticides / |
Author |
Cutkomp, L. K.
|
CORP Author |
Minnesota Univ., St. Paul. Dept. of Entomology, Fisheries and Wildlife. |
Publisher |
Office of Research and Development, U.S. Environmental Protection Agency, For sale by the Supt. of Docs., U.S. Govt. Print. Off., |
Year Published |
1974 |
Report Number |
EPA-660/2-73-027; EPA-R-801029; EPA-16030-ELZ; W74-10526 |
Stock Number |
PB-234 404 |
OCLC Number |
01213615 |
Subjects |
Insecticides--Toxicology ;
Insecticides--Physiological effect ;
Organochlorine compounds ;
Enzymes--Analysis ;
Chlorohydrocarbons ;
Adenosine Triphosphatases--deficiency ;
Hydrocarbons, Chlorinated ;
Insecticides--adverse effects ;
Adenosinetriphosphatase--deficiency ;
Insecticides, Organochlorine--adverse effects
|
Additional Subjects |
Chlorohydrocarbons ;
Insecticides ;
Acaricides ;
Lethal dosage ;
DDT ;
Pesticides ;
Assaying ;
Enzymes ;
Indicator species ;
Toxicology ;
Fishes ;
Brain ;
Phosphorus organic compounds ;
Carbamates ;
Physiological effects ;
Water pollution effects(Animals) ;
Phosphatase/adenosine-tri
|
Internet Access |
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
EJBD |
EPA 660-2-73-027 |
|
Headquarters Library/Washington,DC |
01/12/2015 |
ELBD ARCHIVE |
EPA 660-2-73-027 |
Received from HQ |
AWBERC Library/Cincinnati,OH |
10/04/2023 |
ERAD |
EPA 660/2-73-027 |
|
Region 9 Library/San Francisco,CA |
03/18/2013 |
NTIS |
PB-234 404 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
vi, 39 pages : illustrations ; 27 cm. |
Abstract |
Certain chlorinated hydrocarbon insecticides, especially DDT and closely related chemicals, tested at low concentrations, adversely affect the ATPase enzyme system. DDT inhibited oligomycin-sensitive Mg(2+)ATPase (mitochondrial) both in vitro and in vivo. About 1 micromolar (1 x 10 to the 6th power M) gave 50% inhibition in fish brain and 0.5 parts per billion of DDT in water inhibited about 50% of mitochondrial Mg(2+)ATPase. Na(+)-K(+)ATPase was not inhibited in brain, but was inhibited in vivo in fish gills. Certain discriminating effects were found among chlorinated hydrocarbons, particularly with respect to inhibition of Mg(2+)ATPase, but the ranking of compounds by enzymic effects does not always parallel toxicity values. Organophosphate and carbamate insecticides were ineffective. Further research is needed both in vitro and in vivo to determine how the adverse effects on the enzymes relate to practical interpretations of effects. The abnormally low ATPase activity in chronically treated fish is the first report of an adverse biochemical effect with sublethal doses of DDT. |
Notes |
"Project 16030 ELZ; Program element 1BA027." Includes bibliographical references (pages 37-38). |