||Selective Quenchofluorometric Detection of Fluoranthenic Polycyclic Aromatic Hydrocarbons in High-Performance Liquid Chromatography.
Konash, Pamela L. ;
Wise, Stephen A. ;
May, Willie E. ;
||National Bureau of Standards, Washington, DC.;Environmental Protection Agency, Washington, DC.
Aromatic polycyclic hydrocarbons ;
Chemical analysis ;
High performance liquid chromatography ;
Quenchofluorometric detection systems ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
The phenomenon of fluorescence quenching was used for selective HPLC detection of fluoranthenic polycyclic aromatic hydrocarbons (PAH). Termed a 'Quenchofluorometric' detection system, it employs a filter fluorimeter or spectrofluorimeter and nitromethane in the mobile phase as the fluorescence quenching reagent. Chromatograms obtained with and without the quenching reagent are compared for PAH standards, a coal tar extract, and a shale oil sample. The quenchofluorometric detection system provides an inexpensive method to achieve selective detection for fluoroanthenic PAH as a group.