Main Title |
Histochemical and cytophotometric assay of acid stress in freshwater fish / |
Author |
Anthony, Adam.
|
Other Authors |
|
CORP Author |
Pennsylvania State University. Dept. of Biology. |
Publisher |
United States Environmental Protection Agency, Water Quality Office, |
Year Published |
1971 |
Report Number |
EPA18050-DXJ-05/71; EPA 841-R-71-001; EPA 620-R-41-003 |
OCLC Number |
02695299 |
Subjects |
Acid pollution of rivers, lakes, etc ;
Fishes--Effect of water pollution on ;
Water quality bioassay ;
Fishes--Diseases
|
Additional Subjects |
Acid pollution of rivers, lakes, etc ;
Fishes, Effect of water pollution on ;
Water quality bioassay ;
Fishes--Diseases
|
Internet Access |
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
EJED |
EPA 620-R-71-003 |
|
OCSPP Chemical Library/Washington,DC |
01/12/2018 |
ELBD ARCHIVE |
EPA 620-R-71-003 |
Received from HQ |
AWBERC Library/Cincinnati,OH |
10/04/2023 |
ELBD |
EPA 841-R-71-001 |
|
AWBERC Library/Cincinnati,OH |
10/28/2011 |
|
Collation |
x, 113 pages : illustrations, figures, tables ; 28 cm |
Notes |
"Report date May 1971." Includes bibliographical references (pages 85-92). |
Contents Notes |
The feasibility of using histochemical and histopathological changes in brook trout, longnose dace and fathead minnows as bioindicators of acid pollution was investigated. Laboratory studies entailed using a gravity flow diluter system. Field studies involved using net traps in polluted streams. Exposure durations were 4-5 days and 28-30 days. Histochemical and cytophotometric analyses were made of gills, Stannius corpuscle, blood, spleen, kidney and liver. The primary mode of acid toxicant action is gill damage which results in impaired respiratory, excretory and liver functions. Short term indices of acid stress include: colloidal iron and aminosalicylic acids (PAS) staining of gills and renal Stannius corpuscles. A useful bioindicator of prolonged acid exposure is decreased azure B-RNA staining of liver cells; this assesses the extent of liver impairment and reflects a reduced tolerance of fish to other toxicants. |